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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
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High-throughput Flow-cytometry Measurement of Cellular Mechanotype Based on Rupture and Delivery of DNA Tension Probes into Cells
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Cell-flow technique.

George P Hess, Ryan W Lewis, Yongli Chen

    Cold Spring Harbor Protocols
    |October 3, 2014
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    Summary
    This summary is machine-generated.

    This study introduces a novel cell-flow device for precise neurotransmitter application during electrophysiology. The system ensures constant cell orientation, improving reproducibility in kinetic experiments with ligand-gated ion channels.

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    Area of Science:

    • Neuroscience
    • Biophysics
    • Electrophysiology

    Background:

    • Accurate neurotransmitter application is crucial for studying receptor kinetics.
    • Maintaining constant cell orientation during solution flow is vital for reproducible electrophysiological recordings.
    • Existing devices often struggle with maintaining consistent cell-flow orientation.

    Purpose of the Study:

    • To develop a simple, reliable cell-flow device for rapid solution exchange.
    • To ensure constant cell orientation during perfusion for improved experimental reproducibility.
    • To enable precise, computer-controlled solution application and removal during electrophysiological recordings.

    Main Methods:

    • A peristaltic pump, U-tube, and solenoid valve were integrated into a solution exchange system.
    • The system was designed for rapid (tens of milliseconds) application and removal of solutions.
    • Computer control via a solenoid valve allowed precise timing of solution delivery.

    Main Results:

    • The developed cell-flow device maintains constant cell orientation during solution perfusion.
    • Rapid solution exchange in the tens of milliseconds was achieved.
    • The system facilitated testing multiple conditions on a single cell with buffer washes in between.

    Conclusions:

    • This cell-flow device enhances reproducibility in kinetic experiments by maintaining constant cell orientation.
    • The system offers precise temporal control over solution application for electrophysiological studies.
    • It provides a robust method for investigating receptor function under various solution conditions.