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Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos
Published on: October 9, 2020
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Identification and functional analysis of long non-coding RNAs in mouse cleavage stage embryonic development based on
Kunshan Zhang, Kefei Huang, Yuping Luo1
1Stem Cell Translational Research Center, Tongji Hospital, Tongji University School of Medicine, Shanghai 200092, China. luoyuping@163.com.
BMC Genomics
|October 4, 2014
Summary
This study identifies novel long non-coding RNAs (lncRNAs) in early mouse embryos, revealing their stage-specific expression and roles in embryonic development regulation.
Area of Science:
- Developmental Biology
- Genomics
- Epigenetics
Background:
- Long non-coding RNAs (lncRNAs) are crucial regulators of embryonic development and cell fate.
- Their specific roles and expression profiles in early mammalian development remain largely unexplored.
Purpose of the Study:
- To comprehensively analyze lncRNA profiles in mouse cleavage stage embryos.
- To identify novel lncRNAs and understand their functions in early development.
Main Methods:
- Utilized public single-cell RNA-sequencing data from mouse cleavage stage embryos.
- Reconstructed high-confidence transcripts and identified novel lncRNAs.
- Performed expression profile and co-expression network analyses.
Main Results:
- Identified 5563 novel lncRNAs from 3492 loci, exhibiting characteristics typical of vertebrate lncRNAs.
- Discovered stage-specific expression patterns for many lncRNAs during cleavage stages.
- Co-expression networks linked lncRNAs to cell cycle regulation, transcription, translation, and oxidative phosphorylation.
Conclusions:
- This research presents the first catalog of lncRNAs in mouse cleavage stage embryos.
- Provides insights into the molecular mechanisms governing early embryonic development through lncRNA regulation.
