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Effects of clindamycin and cefuroxime on leukocyte membrane receptors and function
A Noess1, B Hauge, C O Solberg
1Medical Department B, Haukeland Hospital, University of Bergen, Norway.
Abstract:
Normal human granulocytes and lymphocytes were preincubated in 0.5, 5, or 50 mg/l of clindamycin, and 5, 50, or 500 mg/l of cefuroxime. Incubation with clindamycin caused an increase in the proportion of granulocytes bearing receptors for the Fc portion of IgG (Fc gamma-R) and C3b (C3b-R). Random migration in capillary tubes was significantly decreased, but phagocytosis as measured by chemiluminescence was only slightly decreased. The proportion of lymphocytes bearing Fc gamma-R was decreased, while there was no effect on lymphocyte C3b-R percentage, nor on the proportion of granulocytes or lymphocytes bearing sheep erythrocyte (E) receptors. Preincubation of granulocytes in cefuroxime was not associated with changes in the proportion of receptor-bearing cells, except for a slight increase in Fc gamma-R-bearing lymphocytes at the lowest concentration tested. Tube migration was not affected but chemiluminescence was significantly decreased. Preincubation with clindamycin, although increasing the proportion of cells bearing phagocytosis-associated receptors, is thus not associated with an increased phagocytic ability, while cefuroxime incubation caused a decrease in chemiluminescence despite normal levels of receptor-positive cells.
Insights
Clindamycin and cefuroxime impact immune cell receptors and function. Antibiotic exposure altered granulocyte and lymphocyte receptor expression, affecting cell migration and phagocytosis, with clindamycin increasing receptor-bearing cells but not phagocytic ability.
Area of Science:
- Immunology
- Pharmacology
Background:
- Antibiotics can modulate immune cell functions.
- Understanding the effects of clindamycin and cefuroxime on human granulocytes and lymphocytes is crucial for clinical applications.
Purpose of the Study:
- To investigate the in vitro effects of clindamycin and cefuroxime on human granulocyte and lymphocyte receptor expression and function.
- To assess the impact of these antibiotics on cell migration and phagocytosis.
Main Methods:
- Human granulocytes and lymphocytes were incubated with varying concentrations of clindamycin and cefuroxime.
- Receptor expression (Fc gamma-R, C3b-R, E receptors) was analyzed.
- Cell migration (capillary tube assay) and phagocytosis (chemiluminescence) were measured.
Main Results:
- Clindamycin increased Fc gamma-R and C3b-R on granulocytes but decreased random migration and slightly reduced chemiluminescence.
- Clindamycin decreased Fc gamma-R on lymphocytes but did not affect other lymphocyte receptors.
- Cefuroxime did not significantly alter granulocyte receptor expression but decreased chemiluminescence, while slightly increasing Fc gamma-R on lymphocytes at low concentrations.
Conclusions:
- Clindamycin enhances phagocytosis-associated receptor expression on granulocytes without a corresponding increase in phagocytic capacity.
- Cefuroxime impairs granulocyte chemiluminescence despite normal receptor levels.
- These findings highlight differential immunomodulatory effects of clindamycin and cefuroxime on human leukocytes.