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Biodegradation of aliphatic hydrocarbons in the presence of hydroxy cucurbit[6]uril
Rajesh Pasumarthi1, Vikash Kumar2, Sivaraman Chandrasekharan1
1Applied and Environmental Biotechnology Laboratory, Department of Biological Sciences, BITS - Pilani, K K Birla Goa Campus, Zuari Nagar, Goa 403 726, India.
Abstract:
Aliphatic hydrocarbons are one of the major environmental pollutants with reduced bioavailability. The present study focuses on the effect of hydroxy cucurbit[6]uril on the bioavailability of hydrocarbons. A bacterial consortium was used for biodegradation studies under saline and non-saline conditions. Based on denaturing gradient gel electrophoresis results it was found that the consortium under saline conditions had two different strains. The experiment was conducted in microcosms with tetradecane, hexadecane, octadecane and mixture of the mentioned hydrocarbons as the sole carbon source. The residual hydrocarbon was quantified using gas chromatography every 24h. It was found that biodegradation of tetradecane and hexadecane, as individual carbon source increased in the presence of hydroxy CB[6], probably due to the increase in their bioavailability. In case of octadecane this did not happen. Bioavailability of all three aliphatic hydrocarbons was increased when provided as a mixture to the consortium under saline conditions.
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