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Extended blood group molecular typing and next-generation sequencing
Zhugong Liu1, Meihong Liu1, Teresita Mercado1
1Division of Blood Components and Devices, Office of Blood Research and Review, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, MD.
Transfusion Medicine Reviews
|October 5, 2014
Summary
High-throughput blood group molecular typing aids donor-patient matching. Target enrichment next-generation sequencing (NGS) offers comprehensive genetic analysis beyond current platform limitations for improved blood transfusion safety.
Area of Science:
- Genetics
- Immunology
- Transfusion Medicine
Background:
- High-throughput molecular typing platforms predict blood group antigen phenotypes.
- These systems facilitate extended donor/patient matching, including rare alleles.
- Crucial for multiply-transfused patients with alloantibodies.
Purpose of the Study:
- To review current blood group genotyping techniques.
- To discuss the advantages and limitations of existing methods.
- To explore target enrichment next-generation sequencing (NGS) for extended genotyping.
Main Methods:
- Review of multiplex blood group molecular typing platforms.
- Analysis of target enrichment NGS for comprehensive genetic variation detection.
- Comparison of NGS with traditional Sanger sequencing and genotyping platforms.
Main Results:
- Current platforms detect predefined genetic variants but have limitations.
- Target enrichment NGS provides comprehensive sequence data for known and de novo variations.
- NGS detects single-nucleotide polymorphisms, indels, and structural variations.
Conclusions:
- Target enrichment NGS offers a powerful approach for extended blood group genotyping.
- This technology surpasses limitations of current molecular typing methods.
- NGS can enhance the development of advanced blood group genotyping assay systems.
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