Related Experiment Video
Updated: Apr 23, 2026
Enzymatic Modification and Flow Cytometry Assessment of Yeast Surface Displayed Proteins
Published on: May 30, 2025
Capillary electrophoresis with laser-induced fluorescence detection for studying amino acid uptake by yeast during
Heidi Turkia1, Heli Sirén2, Merja Penttilä3
1VTT Technical Research Centre of Finland, PO Box 1000, FIN-02044 VTT, Espoo, Finland; Lappeenranta University of Technology, PO Box 20, FIN-53580 Lappeenranta, Finland.
Abstract:
The amino acid composition of cultivation broth is known to affect the biomass accumulation, productivity, and vitality of yeast during cultivation. A separation method based on capillary electrophoresis with laser-induced fluorescence (LIF) detection was developed for the determination of amino acid consumption by Saccharomyces cerevisiae during beer fermentation. Intraday relative standard deviations were less than 2.1% for migration times and between 2.9% and 9.9% for peak areas. Interday relative standard deviations were less than 2.5% for migration times and between 4.4% and 18.9% for peak areas. The quantification limit was even as low as 62.5 pM which equals to below attomole level detection. The method was applied to study the rate of amino acid utilization during beer fermentation.
