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Direct Detection of the Acetate-forming Activity of the Enzyme Acetate Kinase
Published on: December 19, 2011
Novel anthracene-based fluorescent sensor for selective recognition of acetate anions in protic media
Kuoxi Xu1, Huajie Kong1, Qian Li1
1Institute of Fine Chemical and Engineering, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan 475004, PR China.
Abstract:
Novel 9-substituted anthracene derivatives were synthesized and characterized by IR, HRMS, (1)H and (13)C NMR. The fluorescence titration experiments were explored to study the interaction between the compounds and some anions, such as H2PO4(-), P2O7(4-), F(-), Cl(-), Br(-), I(-), AcO(-) in H2O (0.01 M HEPES, pH=7.4) under imitated physiological conditions. One of these compounds, bearing a phenylalaninol unit, showed specific fluorescence enhancement with acetate anion. The sensor L1 was found to present good selective fluorescence sensing ability to acetate anion through photoinduced electron-transfer mechanism in protic media.

