[Scotchbond dentin-bonding material in the biological test chain--is an adhesive composite-filling technic...

The biological properties of Scotchbond as a dentin-bonding agent were assessed in cytotoxicity tests, subcutaneous implantation, in an acute vital microscopically controlled test on rat incisors and in an usage test on human teeth. The persistent moderate reactions in human teeth in the test period over 90 days, the strong toxicity in the initial and secondary biological test leads to the conclusion, that there is no clinical indication to use the dentin-bonding Scotchbond without proper pulp protection. According to scanning electron microscopy of the marginal adaptation of the enamel etch technique in connection with enamel bonding to long beveled enamel produced acceptable marginal adaptation of P-30 composite restorations.