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Updated: Apr 23, 2026

$On-Chip Crystallization and Large-Scale Serial Diffraction at Room Temperature$

On-Chip Crystallization and Large-Scale Serial Diffraction at Room Temperature

Published on: March 11, 2022

Time-resolved crystallography using the Hadamard transform.

Briony A Yorke¹, Godfrey S Beddard², Robin L Owen³

¹Astbury Centre for Structural Molecular Biology, The University of Leeds, Leeds, UK.

|October 6, 2014

Summary

This summary is machine-generated.

This study introduces a new time-resolved X-ray crystallography method using Hadamard transform, improving signal/noise for studying molecular dynamics. This technique enhances structural dynamics measurements on standard synchrotron beamlines.

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$Microcrystallography of Protein Crystals and In Cellulo Diffraction$

Microcrystallography of Protein Crystals and In Cellulo Diffraction

Published on: July 21, 2017

Area of Science:

Structural Biology
Biophysics
Crystallography

Background:

Time-resolved X-ray crystallography is crucial for understanding molecular dynamics.
Existing pump-probe methods face limitations in speed and signal-to-noise ratio.
High-resolution studies of dynamic processes in proteins and small molecules are needed.

Purpose of the Study:

To present a novel method for time-resolved X-ray crystallographic experiments.
To enhance signal-to-noise ratio compared to traditional pump-probe techniques.
To enable high-resolution measurements of structural dynamics.

Main Methods:

Utilizing the Hadamard transform for time-resolved X-ray crystallography.
Defining time resolution by the periodicity of probe pulse sequences.
Encoding probe signals for enhanced data acquisition.

Main Results:

Achieved significantly improved signal-to-noise ratio over single-pulse pump-probe experiments.
Demonstrated applicability on standard synchrotron beamlines.
Established a method for high-resolution measurements of structural dynamics.

Conclusions:

The Hadamard transform method offers a powerful approach for time-resolved structural dynamics.
This technique is compatible with existing synchrotron infrastructure.
The method has broad applicability to various time-resolved measurements, including spectroscopy.