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Related Experiment Videos

Gamma/delta T cells express a unique surface molecule appearing late during thymic development.

C R Mackay1, M F Beya, P Matzinger

  • 1Basel Institute for Immunology, Switzerland.

European Journal of Immunology
|August 1, 1989
PubMed
Summary

In sheep, gamma/delta T cells, unlike in mice, are abundant and express a unique T19 molecule. T19 marks gamma/delta T cell maturation and function, distinguishing them from other T cells.

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Area of Science:

  • Immunology
  • Cell Biology
  • Veterinary Science

Background:

  • T cells, crucial for adaptive immunity, exist as alpha/beta and gamma/delta subsets.
  • In humans and mice, gamma/delta T cells are a minor subset, typically CD4-CD8-.
  • Sheep exhibit a significantly larger gamma/delta T cell population (30%-60%) compared to mammals.

Purpose of the Study:

  • To characterize the gamma/delta T cell subset in sheep.
  • To identify unique markers and developmental pathways of sheep gamma/delta T cells.
  • To investigate the function and homing patterns of gamma/delta T cells in sheep.

Main Methods:

  • Monoclonal antibody (mAb) against gamma/delta T cells was used for identification.
  • Flow cytometry and immunohistochemistry were employed to analyze cell surface markers (T19, CD45, TcR, MHC class I, CD4, CD8, CD2, LFA-1, CD5).

Related Experiment Videos

  • T cell ontogeny within the thymus and peripheral distribution were studied.

    • Main Results:

    • Sheep gamma/delta T cells express a unique 215 kDa surface molecule, T19, unrelated to CD45 or TcR.
    • T19 expression correlates with gamma/delta T cell maturation stages in the thymus and is associated with Hassall's corpuscles.
    • Peripheral gamma/delta T cells, particularly in the intestinal epithelium, show variable T19 expression, suggesting functional or homing differences.

    Conclusions:

    • The T19 molecule serves as a maturation marker for gamma/delta T cells in sheep.
    • Exclusive T19 expression by gamma/delta T cells suggests a fundamental role in their interactions and functions.
    • Distinctive recirculation patterns of gamma/delta T cells, excluding mesenteric lymph nodes, highlight their specialized immune surveillance roles.