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Related Concept Videos

Plasmodesmata02:32

Plasmodesmata

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The organs in a multicellular organism’s body are made up of tissues formed by cells. To work together cohesively, cells must communicate. One way that cells communicate is through direct contact with other cells. The points of contact that connect adjacent cells are called intercellular junctions.
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Plasmodesmata01:20

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In a multicellular organism, cells must communicate to work together in a coordinated manner. One way that cells communicate is through direct contact with other cells. The points of contact that connect adjacent cells are called intercellular junctions.
Intercellular junctions are a feature of fungal, plant, and animal cells. However, different types of junctions are found in different kinds of cells. Intercellular junctions found in animal cells include tight junctions, gap junctions, and...
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Updated: Apr 23, 2026

A Strategy to Validate the Role of Callose-mediated Plasmodesmal Gating in the Tropic Response
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Probing plasmodesmata function with biochemical inhibitors.

Rosemary G White1

  • 1Black Mountain Laboratories, CSIRO Plant Industry, GPO Box 1600, Clunies Ross Street, Black Mountain, ACT, 2601, Australia, rosemary.white@csiro.au.

Methods in Molecular Biology (Clifton, N.J.)
|October 8, 2014
PubMed
Summary
This summary is machine-generated.

Investigating plasmodesmata (PD) function involves using inhibitors to study cell-to-cell transport. Researchers monitor changes in fluorescent dye movement and PD structure using techniques like Transmission Electron Microscopy (TEM).

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Area of Science:

  • Plant Biology
  • Cell Biology
  • Biochemistry

Background:

  • Plasmodesmata (PD) are crucial for intercellular communication in plants.
  • Understanding PD function is key to plant physiology and development.
  • Current methods for studying PD often involve indirect measurements.

Purpose of the Study:

  • To outline a comprehensive approach for investigating plasmodesmata (PD) function.
  • To detail methods for assessing the impact of inhibitors on cell-to-cell transport.
  • To describe techniques for evaluating PD structure alterations.

Main Methods:

  • Application of specific inhibitors targeting physiological processes or cellular components.
  • Monitoring cell-to-cell transport using fluorescent tracer dyes or free fluorescent proteins.
  • Analyzing PD structure via Transmission Electron Microscopy (TEM) on embedded tissue sections.

Main Results:

  • Inhibitor application effectively modulates cell-to-cell transport through plasmodesmata.
  • Fluorescent tracers and proteins provide quantifiable measures of transport changes.
  • TEM reveals structural modifications in plasmodesmata in response to treatments.

Conclusions:

  • Inhibitor-based studies are a valuable tool for dissecting plasmodesmata function.
  • Combining transport assays with structural analysis offers robust insights into PD.
  • Careful consideration of experimental caveats is essential for accurate interpretation.