Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Introduction to Nuclear Reprogramming01:14

Introduction to Nuclear Reprogramming

1.3K
Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
1.3K
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

14.8K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
14.8K
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

1.4K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
1.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Yeast nuclei-mediated precise delivery of synthetic megabase-scale human DNA into mammalian embryos.

Nature protocols·2026
Same author

TFPI2 promotes NK cell-mediated glioblastoma killing through adhesion and checkpoint control.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Engineering carbon nanofiber-supported NiCo/CoNi<sub>2</sub>S<sub>4</sub> Mott-Schottky heterostructure with robust interfacial electric field for boosting oxygen evolution reaction kinetics.

Journal of colloid and interface science·2026
Same author

Optic chiasmal neuritis: clinical features, aetiologies, MRI patterns and prognosis in a real-world cohort.

Journal of neurology·2026
Same author

Early corticosteroids predict visual whereas MRI lesion extent predicts retinal atrophy outcomes in MOG antibody-associated optic neuritis.

Multiple sclerosis (Houndmills, Basingstoke, England)·2026
Same author

HSPA6 is induced by RIG-I-like receptors and negatively regulates type-I interferon signaling.

Cellular and molecular life sciences : CMLS·2026
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Apr 23, 2026

Nuclear Transfer into Mouse Oocytes
14:17

Nuclear Transfer into Mouse Oocytes

Published on: November 30, 2006

18.0K

Nuclear transfer in the mouse.

Vincent Brochard1, Zichuan Liu

  • 1INRA, UMR1198 Biologie du Développement et Reproduction, Jouy-en-Josas, 78350, France.

Methods in Molecular Biology (Clifton, N.J.)
|October 8, 2014
PubMed
Summary
This summary is machine-generated.

Nuclear transfer (NT) is a powerful technique for reprogramming and deriving embryonic stem cells. The described "one step method" efficiently combines nuclear injection and enucleation for mouse cloning and micromanipulation.

More Related Videos

Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT
13:36

Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT

Published on: September 30, 2010

14.8K
Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer
09:52

Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer

Published on: April 26, 2018

15.5K

Related Experiment Videos

Last Updated: Apr 23, 2026

Nuclear Transfer into Mouse Oocytes
14:17

Nuclear Transfer into Mouse Oocytes

Published on: November 30, 2006

18.0K
Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT
13:36

Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT

Published on: September 30, 2010

14.8K
Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer
09:52

Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer

Published on: April 26, 2018

15.5K

Area of Science:

  • Developmental Biology
  • Reproductive Technology
  • Stem Cell Biology

Background:

  • Nuclear transfer (NT) is a key technique for studying cellular reprogramming and deriving nuclear transfer-embryonic stem (ntES) cells.
  • The Piezo-driven direct microinjection method is a validated approach for cloning mice and other species, widely used in mouse micromanipulation.

Purpose of the Study:

  • To describe the
  • one step method
  • protocol for nuclear transfer in mice.
  • To detail a streamlined procedure combining nuclear injection and oocyte enucleation in a single step.

Main Methods:

  • Isolation and collection of mouse oocytes.
  • Preparation and treatment of donor cells.
  • Visualization of the spindle-chromosomal complex.
  • Direct injection of donor nucleus and simultaneous enucleation of the oocyte.
  • Activation of reconstructed embryos.
  • In vitro culture and transfer of embryos into pseudopregnant mice.

Main Results:

  • The described protocol provides a comprehensive method for mouse nuclear transfer.
  • The
  • one step method
  • simplifies the nuclear transfer procedure by combining key steps.

Conclusions:

  • The "one step method" offers an efficient and versatile protocol for mouse nuclear transfer.
  • This technique is valuable for studying reprogramming, deriving ntES cells, and advancing mouse micromanipulation applications.