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Related Concept Videos

Subviral Agents01:29

Subviral Agents

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Subviral agents are infectious entities that resemble viruses but lack one or more viral components, such as a capsid or essential replication machinery. These agents include viroids, prions, and satellites, each possessing distinct structural and functional characteristics that influence their mode of infection and replication.Viroids are the simplest subviral agents, consisting of circular, single-stranded RNA molecules without a protein coat. They exclusively infect plants, relying entirely...
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RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
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Efficient double-stranded RNA production methods for utilization in plant virus control.

Andreas E Voloudakis1, Maria C Holeva, L Peter Sarin

  • 1Laboratory of Plant Breeding and Biometry, Agricultural University of Athens, 75 Iera Odos, Athens, 11855, Greece, avoloud@aua.gr.

Methods in Molecular Biology (Clifton, N.J.)
|October 8, 2014
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Summary
This summary is machine-generated.

Double-stranded RNA (dsRNA) triggers RNA silencing (RNAi) to protect plants from viruses. This review details cost-effective dsRNA production methods for crop protection applications.

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Area of Science:

  • Plant Pathology
  • Molecular Biology
  • Biotechnology

Background:

  • RNA silencing (RNAi) is a conserved eukaryotic pathway controlling gene expression.
  • Double-stranded RNA (dsRNA) initiates RNAi, leading to sequence-specific degradation of target RNA.
  • This mechanism provides resistance against pathogens, particularly viruses.

Purpose of the Study:

  • To review methods for producing double-stranded RNA (dsRNA).
  • To highlight the potential of dsRNA as a crop protection agent.
  • To address the growing demand for cost-effective dsRNA production.

Main Methods:

  • Detailed description of in vitro dsRNA production techniques.
  • Detailed description of in vivo dsRNA production techniques.
  • Discussion of enzymatic synthesis of dsRNA molecules.

Main Results:

  • dsRNA application directly to plant tissues can induce virus resistance.
  • Transgenic plants expressing dsRNA show resistance to viral infections.
  • Various methods for dsRNA synthesis are available for agricultural applications.

Conclusions:

  • dsRNA is a potent tool for inducing sequence-specific gene silencing in plants.
  • dsRNA-mediated resistance offers a promising strategy for viral disease management in crops.
  • Development of efficient and cost-effective dsRNA production is crucial for agricultural implementation.