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Updated: Feb 10, 2026

Virus-induced Gene Silencing VIGS in Nicotiana benthamiana and Tomato
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Control Strategies of Plant Viruses Using Spray-Induced Gene Silencing.

Seung-Kook Choi1, Eseul Baek2, Ho-Jong Ju3

  • 1Virology Unit, Department of Horticultural and Herbal Environment, National Institute of Horticultural and Herbal Science, RDA, Wanju 55365, Korea.

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|February 9, 2026
PubMed
Summary

Spray-induced gene silencing (SIGS) uses double-stranded RNA (dsRNA) to protect crops from viruses. Advances in dsRNA production and formulation show promise for sustainable agriculture, but challenges remain.

Keywords:
RNA interferencecontroldouble-strand RNAplant virusesspray-induced gene silencing

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Area of Science:

  • Agricultural science
  • Molecular biology
  • Biotechnology

Background:

  • Plant viruses cause significant crop losses globally.
  • Existing antiviral controls are limited, lacking pesticide-like efficacy.
  • RNA interference (RNAi) offers a sequence-specific, non-transgenic approach to antiviral defense.

Purpose of the Study:

  • To review the technological advancements and challenges of spray-induced gene silencing (SIGS) for plant virus management.
  • To highlight the integration of nanotechnology and field application methodologies in SIGS.
  • To assess the potential of dsRNA technology for sustainable crop protection.

Main Methods:

  • Review of recent scientific literature on dsRNA production, formulation, and application.
  • Analysis of industry-scale dsRNA manufacturing and nanomaterial-based delivery systems.
  • Evaluation of SIGS advantages, challenges, and future research directions.

Main Results:

  • SIGS utilizes externally produced dsRNA via foliar sprays, seed treatments, or root uptake for virus suppression.
  • Industry-scale dsRNA production and advanced formulations enhance stability, uptake, and persistence in plants.
  • Key challenges include environmental degradation, limited systemic movement, production costs, and regulatory hurdles.

Conclusions:

  • SIGS represents a promising, non-transgenic strategy for sustainable plant virus management.
  • Innovations in RNA production, formulation, and delivery are crucial for overcoming current limitations.
  • Integrating SIGS with nanotechnology and robust field application methods positions it as a transformative technology for next-generation crop protection.