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Related Concept Videos

Asthma I: Introduction01:28

Asthma I: Introduction

40
Asthma is a chronic inflammatory disorder of the airways characterized by variable airflow obstruction and heightened bronchial responsiveness to a wide range of triggers. The underlying inflammation leads to airway swelling, mucus hypersecretion, and smooth muscle constriction, all of which narrow the airway lumen and impede airflow. Clinically, asthma presents with recurrent episodes of wheezing, shortness of breath, chest tightness, and coughing, symptoms that typically vary in intensity and...
40
Asthma-II: Pathophysiology and Classification01:26

Asthma-II: Pathophysiology and Classification

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Asthma is a prevalent chronic respiratory condition marked by inflammation and hyperresponsiveness of the airways. Its pathophysiology involves complex interactions among inflammatory pathways, immune responses, and neural mechanisms.
Additionally, environmental and genetic factors play crucial roles in determining an individual's susceptibility to asthma and the severity of their condition.
Critical processes in asthma pathophysiology include:
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Asthma: Pathogenesis and Management01:20

Asthma: Pathogenesis and Management

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Asthma is a chronic pulmonary condition involving inflammation of the airways, hyper-reactivity, and reversible obstruction of the airways. This condition can significantly impact a person's quality of life, making breathing difficult and leading to distressing symptoms.
Asthma is classified as allergic and non-allergic. Allergens such as dust mites, pollen, and pet dander trigger allergic asthma, while factors like cold air, intense emotions, or exercise can induce non-allergic asthma.
1.5K
Asthma III: Clinical Manifestations01:13

Asthma III: Clinical Manifestations

25
Asthma presents with a characteristic pattern of episodic respiratory symptoms that reflect underlying airway inflammation, bronchoconstriction, and mucus hypersecretion. Although severity varies among individuals, certain clinical manifestations are considered hallmarks of the disorder and often guide diagnosis and assessment.Respiratory SymptomsA persistent cough is one of the most common early features of asthma. It is frequently dry and tends to worsen at night or in the early morning,...
25
Asthma-I: Introduction01:29

Asthma-I: Introduction

3.5K
Asthma is a chronic respiratory ailment that requires careful management due to its varying symptoms and influencing factors. It is characterized by airway inflammation, bronchial hyperresponsiveness, and reversible airflow obstruction, leading to symptoms like wheezing, shortness of breath, chest tightness, and coughing. The symptom frequency and intensity may vary considerably over time. It is also linked to immune system responses to allergens and irritants, highlighting the complex...
3.5K
Asthma-IV: Diagnostic and Management01:30

Asthma-IV: Diagnostic and Management

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The diagnosis and management of asthma are comprehensive, encompassing clinical assessments, lung function tests, and pharmacological interventions. Here's an overview:
Clinical Assessment for Asthma:
This is the first step in diagnosing and managing asthma. It includes:
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Identification and Characterization of Immunogenic RNA Species in HDM Allergens that Modulate Eosinophilic Lung Inflammation
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Epigenome variation in severe asthma.

Kenneth Wysocki1, Yvette Conley2, Sally Wenzel3

  • 1School of Nursing, University of Pittsburgh, Pittsburgh, PA, USA ken.wysocki@me.com.

Biological Research for Nursing
|October 8, 2014
PubMed
Summary
This summary is machine-generated.

Epigenetics, specifically DNA methylation, shows significant differences between severe asthma patients and healthy individuals. This pilot study highlights potential new biomarkers for severe asthma using genome-wide methylation profiling.

Keywords:
epigeneticgenemethylationsevere asthma

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Area of Science:

  • Epigenetics and Genomics
  • Molecular Biology
  • Respiratory Medicine

Background:

  • Asthma is a complex disease with varying severity.
  • Genomics has advanced asthma understanding, but epigenetics, influenced by environmental factors, is a key emerging area.
  • Epigenetic mechanisms like DNA methylation may explain gene expression variations in asthma.

Purpose of the Study:

  • To detail the methodology for genome-wide methylation profiling in severe asthma.
  • To present epigenomic data from a cohort with severe asthma.
  • To evaluate data collection and analysis techniques for epigenomic studies.

Main Methods:

  • Analyzed methylation status of over 14,000 gene promoter sites.
  • Utilized the Illumina Infinium HumanMethylation27BeadChip platform for DNA methylation analysis.
  • Compared six severe asthma subjects with four healthy controls, using blood samples and technical replicates.

Main Results:

  • High concordance was observed in technical replicates.
  • Statistically significant differences in DNA methylation profiles were found between severe asthma patients and controls (p < 10(-8)).
  • Three gene promoter regions (FAM181A, ZNF718, MRI1) remained significant after multiple testing correction.

Conclusions:

  • The Illumina platform demonstrates internal validity for methylation analysis in stored blood DNA.
  • Significant methylation differences suggest a role for epigenetics in severe asthma pathogenesis.
  • Further research with larger sample sizes is needed to validate these epigenomic findings.