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Related Experiment Video

Updated: Apr 22, 2026

Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells

Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells

Published on: February 2, 2013

The single-cell assay in cell-mediated cytotoxicity.

B Bonavida¹, T P Bradley¹, E A Grimm²

¹Department of Microbiology and Immunology, UCLA School of Medicine, Los Angeles, CA 90024, USA.

Immunology Today

|October 8, 2014

Summary

A new agarose assay enables the study of single cytotoxic cells, advancing cellular immunology research. This method allows direct enumeration of individual effector cells, similar to previous advances in antibody-producing cell studies.

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Last Updated: Apr 22, 2026

Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells

Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells

Published on: February 2, 2013

Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity

Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity

Published on: December 17, 2013

Droplet-based Cytotoxicity Assay to Assess Chimeric Antigen Receptor T cells at the Single-cell Level

Droplet-based Cytotoxicity Assay to Assess Chimeric Antigen Receptor T cells at the Single-cell Level

Published on: March 14, 2025

Area of Science:

Immunology
Cellular Biology
Assay Development

Background:

The Jerne plaque assay revolutionized humoral immunity studies by enabling direct enumeration of antibody-producing plasma cells.
Cellular cytotoxicity research was previously limited to analyzing bulk effector cell populations, hindering detailed single-cell analysis.

Purpose of the Study:

To describe a novel agarose-based assay for the study of single cytotoxic cells.
To provide a method for direct enumeration and analysis of individual effector cells in cellular cytotoxicity.

Main Methods:

Development of an assay in agarose for the study of single cytotoxic cells.
Adaptation of principles from cell enumeration assays for application to cytotoxic effector cells.

Main Results:

The described assay allows for the direct study and enumeration of individual cytotoxic cells.
This method overcomes previous limitations in analyzing cellular cytotoxicity at the single-cell level.

Conclusions:

The new agarose assay significantly advances the study of cellular cytotoxicity.
This technique facilitates detailed investigation of individual effector cell function and characterization.