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Rosette tests for T and B lymphocyte immunoglobulin
1Department of Pathology, Montreal General Hospital and McGill University Montreal, Canada.
Immunology Today
|October 8, 2014
Summary
This study reviews antiglobulin rosette tests for detecting lymphocyte immunoglobulin (Ig) and Ig-related determinants. These methods, including MARR and DARR, offer advantages over direct binding techniques for identifying surface molecules.
Area of Science:
- Immunology
- Cell Biology
Background:
- B lymphocytes utilize surface immunoglobulin (Ig) as antigen receptors.
- The nature of T lymphocyte antigen receptors is debated, but they show reactivity with anti-Ig reagents, suggesting an Ig-related structure.
Purpose of the Study:
- To review and compare antiglobulin rosette tests, specifically the mixed antiglobulin rosetting reaction (MARR) and direct antiglobulin rosetting reaction (DARR).
- To evaluate these methods for demonstrating lymphocyte Ig and Ig-related surface determinants.
- To compare rosetting techniques with conventional direct binding methods.
Main Methods:
- Review of antiglobulin rosette tests (MARR and DARR).
- Comparison with direct binding methodology using free anti-Ig molecules.
Main Results:
- Antiglobulin rosette tests, particularly MARR and DARR, are highlighted for their utility in detecting lymphocyte surface determinants.
- These rosetting techniques offer special merit compared to conventional direct binding assays.
Conclusions:
- Antiglobulin rosette assays provide valuable methods for the demonstration of lymphocyte immunoglobulin and related surface molecules.
- MARR and DARR are effective alternatives to direct binding for characterizing lymphocyte surface antigens.
