Related Experiment Video
Updated: Apr 22, 2026
09:47
Generation of Human Alloantigen-specific T Cells from Peripheral Blood
Published on: November 21, 2014
12.4K
The cloning of T lymphocytes
1Laboratory of Immunology, MAID, National Institutes of Health, Bethesda, MD 20205, U.S.A.
Immunology Today
|October 8, 2014
Summary
Researchers can now isolate specific T cell populations, revolutionizing cellular immunology research and clinical applications. This breakthrough enables deeper understanding of T-cell specificity and antigen recognition.
Area of Science:
- Cellular Immunology
- Molecular Biology
- Immunogenetics
Background:
- T cells play a crucial role in adaptive immunity.
- Understanding T cell specificity is vital for immunology.
- Previous methods limited the study of specific T cell populations.
Purpose of the Study:
- To outline methods for cloning T lymphocytes.
- To discuss the advantages and disadvantages of T cell cloning techniques.
- To highlight recent applications of T cell cloning in understanding T cell specificity.
Main Methods:
- Isolation of monoclonal T cell populations.
- Utilizing antigen-specific T cell cloning.
- Reviewing established and novel cloning approaches.
Main Results:
- Demonstrated feasibility of isolating antigen-specific T cells.
- Provided a comparative analysis of different cloning techniques.
- Showcased new insights into T cell recognition mechanisms.
Conclusions:
- T cell cloning represents a significant advancement in cellular immunology.
- This technology offers vast potential for basic research and clinical medicine.
- Further exploration of T cell specificity is facilitated by these cloning methods.
Related Concept Videos
01:23Cells of the Adaptive Immune Response
6.7K
The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
6.7K
01:22T Cell Activation and Clonal Selection
13.5K
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Naive T cells that have not yet encountered an antigen express two primary CD...
13.5K
01:27Reproductive Cloning
28.4K
Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic...
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic...
28.4K
01:08Cloning of Dolly the Sheep
8.5K
The first successfully cloned mammal was Dolly, a sheep, born on 5th July 1996 at Roslin Institute, Scotland. The cloned sheep was named after the American singer Dolly Parton. Dolly lived for seven years and died of respiratory complications, which is speculated to be due to the actual age of her DNA. Because the DNA in cloned cells belongs to an older individual, the cloned individual’s life expectancy may be affected. Indeed, analysis of Dolly’s DNA revealed shorter...
8.5K
01:14Introduction to Nuclear Reprogramming
1.3K
Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
1.3K