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Bioluminescence resonance energy transfer (BRET) to detect the interactions between kappa opioid receptor and non
1Department of Pharmacy and Biotechnology (FaBiT), University of Bologna, Irnerio, 48, Bologna, 40126, Italy, andrea.bedini@unibo.it.
Abstract:
Bioluminescence resonance energy transfer (BRET) is a very sensitive technique employed to study protein-protein interactions, including G-protein-coupled receptors (GPCRs) hetero- and homo-dimerization. Recently, BRET has also been used to investigate the interaction between GPCRs (e.g., β2 adrenergic receptor, muscarinic M2 receptor, dopaminergic D2 receptor) and non-visual arrestins. Here a BRET protocol is described to investigate interactions between the kappa opioid receptor (KOR) and non visual arrestins (arrestin-2 and arrestin-3) in HEK-293 cells, both under basal conditions and after exposure to KOR ligands.
