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Updated: Apr 22, 2026

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries
Published on: February 25, 2016
Hepatic inducible nitric oxide synthase expression increases upon exposure to hypergravity.
1Department of Pathology and Translational Genomics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea.
Exposure to hypergravity significantly increases inducible nitric oxide synthase (iNOS) in mouse livers. This upregulation occurs at both the gene transcription and protein expression levels, peaking at 12 hours post-exposure.
Area of Science:
- Hepatology
- Physiology
- Molecular Biology
Background:
- Inducible nitric oxide synthase (iNOS) is upregulated by various stressors in hepatocytes.
- Preliminary observations indicated hypergravity might increase hepatic iNOS gene transcription.
Purpose of the Study:
- Confirm preliminary findings on hypergravity-induced iNOS upregulation.
- Investigate the temporal distribution of iNOS protein in mouse livers under hypergravity conditions.
Main Methods:
- ICR mice were exposed to +3 Gz for 1 hour.
- Time course analysis of iNOS mRNA and protein expression in liver tissue.
- Immunohistochemical detection of iNOS protein localization and intensity.
Main Results:
- Hepatic iNOS mRNA levels significantly increased from 3 to 12 hours post-hypergravity exposure, peaking at 12 hours.
- iNOS protein expression patterns mirrored mRNA changes, with increased cytoplasmic immunoreactivity observed in hepatocytes.
- Maximal iNOS-positive hepatocyte counts and strong expression intensity were noted at 12 hours.
Conclusions:
- Hypergravity exposure significantly upregulates inducible nitric oxide synthase (iNOS) in mouse livers.
- This upregulation occurs at both transcriptional (mRNA) and translational (protein) levels.
- The findings suggest a significant physiological response of hepatocytes to hypergravity stress.
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