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Monitoring Intraspecies Competition in a Bacterial Cell Population by Cocultivation of Fluorescently Labelled Strains
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A model for sigma factor competition in bacterial cells.

Marco Mauri1, Stefan Klumpp1

  • 1Max Planck Institute of Colloids and Interfaces, Potsdam, Germany.

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|October 10, 2014
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Summary
This summary is machine-generated.

Sigma factor competition regulates bacterial gene expression by modulating RNA polymerase (RNAP) holoenzyme availability. This competition influences transcription, especially at overlapping promoters, and plays a role in global gene expression switches.

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Area of Science:

  • Bacterial molecular biology
  • Systems biology
  • Theoretical biology

Background:

  • Sigma factors are essential for bacterial gene expression, controlling global genetic switches.
  • Sigma factors compete for RNA polymerase (RNAP) core enzymes, enabling cross-talk between gene classes.

Purpose of the Study:

  • To develop and validate a theoretical model analyzing sigma factor competition's impact on global gene expression.
  • To investigate how RNAP availability and transcription affect sigma factor competition.

Main Methods:

  • Development of a theoretical model for sigma factor-RNAP binding, transcription, and component availability.
  • Validation of the model using in vitro competition experiments.
  • Analysis of sigma factor competition under various conditions, including the stringent response.

Main Results:

  • The model accurately predicts in vitro competition experiments.
  • Sigma factor competition affects transcription by modulating holoenzyme concentrations, with saturated promoters being less affected unless promoters overlap.
  • Active transcription reduces sigma factor-RNAP affinity, creating complex cross-talk; non-specific DNA binding has minimal impact.

Conclusions:

  • Sigma factor competition is a key mechanism influencing bacterial gene expression.
  • Passive control, facilitated by sigma factor competition, plays a significant role in global gene expression program switches, such as during the stringent response.