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Updated: Jun 18, 2026
06:29
Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells
Published on: January 29, 2014
Immune complex modulation by plasma proteins. With special reference to the complement system and autoimmune
1Institute of Medical Microbiology, University of Odense.
Danish Medical Bulletin
|October 1, 1989
Summary
The complement system
Area of Science:
- Immunology
- Biochemistry
Background:
- The complement (C) system, comprising classical and alternative pathways, interacts with immune complexes (IC).
- C activation by IC influences IC properties, affecting solubility, phagocytosis, and inflammation.
- Dysregulated IC-C interaction can lead to chronic inflammatory diseases.
Purpose of the Study:
- To review aspects of IC-C interactions.
- To develop and characterize an assay for measuring C activity in solubilizing fluid-phase IC (CMS assay).
- To investigate the correlation between CMS capacity, disease activity, and C factors in patients with systemic inflammatory diseases.
Main Methods:
- Development of a complement-mediated solubilization (CMS) assay for fluid-phase IC.
- Characterization of the CMS assay by varying C deficiencies, divalent metal ions, temperature, and ionic strength.
- Analysis of serum from patients with systemic lupus erythematosus (SLE) and other inflammatory conditions using the CMS assay.
- Investigation of potential CMS inhibitors in SLE patient sera using HPLC.
Main Results:
- The CMS assay is dependent on the alternative C pathway and facilitated by the classical pathway.
- Serum from SLE and other inflammatory disease patients showed reduced IC solubilization capacity (CMS).
- Reduced CMS capacity correlated inversely with disease activity in SLE patients.
- A novel CMS inhibitor was identified in some SLE sera, potentially linked to incompletely solubilized IC.
Conclusions:
- The CMS assay effectively measures C activity in IC solubilization.
- Reduced CMS capacity and presence of inhibitors may contribute to IC-mediated inflammatory diseases like SLE.
- Further research into CMS inhibitors and their role in SLE pathogenesis is warranted.
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