Related Experiment Video
Updated: Apr 22, 2026
06:46
Collection of Alfalfa Root Exudates to Study the Impact of Di2-ethylhexyl Phthalate on Metabolite Production
Published on: June 2, 2023
2.4K
Alfalfa (Medicago sativa L.)
Chunxiang Fu1, Timothy Hernandez, Chuanen Zhou
1Forage Improvement Division, The Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK, 73401, USA.
Methods in Molecular Biology (Clifton, N.J.)
|October 11, 2014
Summary
This study presents an efficient method for creating numerous transgenic alfalfa plants using sonication-assisted Agrobacterium-mediated transformation. The protocol yields over 80% success in generating rooted plants within 4-5 months.
Area of Science:
- Plant Biotechnology
- Molecular Biology
- Agricultural Science
Background:
- Alfalfa (Medicago sativa L.) is a crucial forage crop globally.
- Efficient methods for genetic modification are vital for crop improvement.
Purpose of the Study:
- To describe an efficient protocol for generating a large number of transgenic alfalfa plants.
- To utilize sonication-assisted Agrobacterium-mediated transformation for alfalfa genetic engineering.
Main Methods:
- Employing binary vectors with selectable marker genes (bar, npt II, hph).
- Sterilizing intact trifoliates from greenhouse-grown alfalfa.
- Inoculating leaf tissues with Agrobacterium strain EHA105.
- Utilizing sonication to enhance transformation efficiency.
Main Results:
- Successful generation of transgenic alfalfa plants.
- Over 80% of infected leaf pieces produced rooted transgenic plants.
- Transformation process completed within 4-5 months.
Conclusions:
- Sonication-assisted Agrobacterium-mediated transformation is an effective method for producing transgenic alfalfa.
- This protocol facilitates large-scale generation of genetically modified alfalfa for research and agriculture.
Related Concept Videos
01:29Drugs for Treatment of Ulcerative Colitis in IBD
689
Ulcerative colitis is a chronic inflammatory condition primarily affecting the colon and rectum. The primary drugs used in the treatment of ulcerative colitis are aminosalicylates. They exhibit anti-inflammatory and immunosuppressive properties. They modulate inflammatory mediators and inhibit the activity of nuclear factor κB (NF-κB). Aminosalicylates also reduce inflammation by inhibiting prostaglandin and leukotriene production and decreasing neutrophil chemotaxis and superoxide...
689
01:21Green Algae
1.1K
Green algae, also referred to as chlorophytes, are different from red algae in having the chloroplasts containing chlorophylls a and b, which give them their distinct green hue. However, they lack phycobiliproteins, preventing them from developing the red or blue-green pigmentation seen in red algae. In terms of photosynthetic pigment composition, green algae closely resemble plants and share a close evolutionary relationship with them. Taxonomically Green algae belong to Phylum Chlorophyta in...
1.1K
01:19Other Algae
618
The group Stramenopiles include some phototrophic microorganisms. Members of this group possess flagella covered in numerous short, hairlike extensions, a feature that inspired the group's name, derived from the Latin words for "straw" and "hair." Some of the main categories of Stramenopiles include diatoms, golden algae, and brown algae.Diatoms are unicellular, photosynthetic eukaryotes, with over 200 known genera. They play a key role in the planktonic communities of both marine and...
618
01:40Overview of Metabolism
25.5K
Living cells constantly carry out various chemical reactions which are necessary for their proper functioning. These reactions are interlinked to one another via multiple pathways. The collection of these chemical reactions is known as metabolism.
Plant Metabolism
Sunlight, the primary source of energy in plants, is first absorbed by the chlorophyll pigments present in their leaves. Plants then use this energy to carry out photosynthesis, where water is oxidized into oxygen and carbon dioxide...
Plant Metabolism
Sunlight, the primary source of energy in plants, is first absorbed by the chlorophyll pigments present in their leaves. Plants then use this energy to carry out photosynthesis, where water is oxidized into oxygen and carbon dioxide...
25.5K
01:23Red Algae
1.9K
Red algae, also known as rhodophytes, are primarily found in marine environments, though some species inhabit freshwater and terrestrial ecosystems. These organisms exist in both unicellular and multicellular forms, with some multicellular varieties reaching macroscopic sizes.As phototrophic organisms, red algae contain chlorophyll a; however, their chloroplasts lack chlorophyll b. Instead, they possess phycobiliproteins, which serve as major light-harvesting pigments, similar to those found in...
1.9K
03:06Alkali Metals
17.9K
Group 1 elements are soft and shiny metallic solids. They are malleable, ductile, and good conductors of heat and electricity. The melting points of the alkali metals are unusually low for metals and decrease going down the group, while the density increases going down the group with the exception of potassium (Table 1).
Table 1: Properties of the alkali metals
Table 1: Properties of the alkali metals
17.9K