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Simple and rapid purification of F1-ATPase from mitochondria
Y Shinohara1, M Nakagawa, H Terada
1Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.
FEBS Letters
|October 9, 1989
Abstract:
A simple, rapid method for isolation of F1-ATPase from bovine heart mitochondria using the cation exchange resin Dynospheres PD-201-SCX was developed. The method is based on the fact that hydrophobic membrane proteins are adsorbed efficiently on this resin, whereas hydrophilic proteins, such as F1-ATPase, are not. By this method F1-ATPase of high purity and enzyme activity can be obtained from mitochondria in about 2 h.