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Immunofluorescence Microscopy

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.

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Related Experiment Video

Updated: Apr 22, 2026

Fluorescence-mediated Tomography for the Detection and Quantification of Macrophage-related Murine Intestinal Inflammation

Fluorescence-mediated Tomography for the Detection and Quantification of Macrophage-related Murine Intestinal Inflammation

Published on: December 15, 2017

Macrophages shine bright.

Oliver Soehnlein¹

¹LUDWIG MAXIMILIAN UNIVERSITY OF MUNICH; AMSTERDAM UNIVERSITY; GERMAN CENTRE FOR CARDIOVASCULAR RESEARCH.

|October 11, 2014

Summary

Researchers developed a new mouse model for studying macrophage biology. This model uses green fluorescence protein (GFP) to track monocytes and macrophages, overcoming limitations of previous models.

Area of Science:

Immunology and Cell Biology
Transgenic Animal Models
Macrophage Biology

Background:

Studying macrophage populations (monocytes, tissue-resident, and inflammatory macrophages) is crucial for understanding immune responses.
Existing transgenic mouse models like CCR2-RFP and CX3CR1GFP have limitations due to chemokine receptor manipulation.
These limitations can affect monocyte dynamics and fluorescent protein expression, complicating research.

Purpose of the Study:

To create a novel mouse model for enhanced visualization and tracking of macrophage populations.
To provide a reliable tool for future research in macrophage biology and function.
To overcome the limitations associated with existing chemokine receptor-based fluorescent reporter mouse models.

Main Methods:

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Last Updated: Apr 22, 2026

Fluorescence-mediated Tomography for the Detection and Quantification of Macrophage-related Murine Intestinal Inflammation

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Published on: December 15, 2017

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Development of a transgenic mouse model with strong green fluorescence protein (GFP) expression.

Targeted expression of GFP in monocytes, tissue-resident macrophages, and inflammatory macrophages.

Comparison with existing CCR2-RFP and CX3CR1GFP mouse models to highlight advantages.

Main Results:

Successful creation of a novel mouse model with robust GFP expression in key myeloid cell populations.
Demonstration of the model's potential utility for tracking macrophage populations in vivo.
Identification of limitations in previous models related to chemokine receptor knock-in strategies.

Conclusions:

The novel GFP-expressing mouse model offers a valuable tool for macrophage research.
This model circumvents issues related to chemokine receptor alterations seen in other reporter mice.
It facilitates more accurate studies of macrophage biology, recruitment, and function.