Related Experiment Video
Updated: Jun 17, 2026

Transfer of Manipulated Tumor-associated Neutrophils into Tumor-Bearing Mice to Study their Angiogenic Potential In Vivo
Published on: July 20, 2019
Neutrophil regulation of immunotherapy for cancer is controlled by type II interferon
Shengduo Pei1, Yueyun Pan1, Heng Liang1
1Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden.
Abstract:
Tumor resistance to immunotherapy is driven by several mechanisms, including those imposed by myeloid populations. Neutrophils are prominent within this landscape and display functional heterogeneity. Here, we investigated the contextual role of neutrophils, and using neutropenic mice, we found that the dominating function was to block the response when targeting T cells or myeloid cells. We found that neutrophils upregulated programmed death ligand-1 (PD-L1) in response to the treatment and, using this as a target, depleted this population. The upregulation of PD-L1 was dependent on interferon-γ (IFN-γ) produced by cytotoxic lymphocytes. Specific genetic deletion of cd274 or Ifngr1 on neutrophils showed that this was cell intrinsic. Moreover, in the absence of the capacity for specific IFN-γ-driven suppression, neutrophils changed their phenotype to support immunotherapy. Thus, we find that the type II interferon, IFN-γ, is key in determining whether neutrophils will support or block immunotherapy for cancer.
More Related Videos
Related Concept Videos
Tumor Immunotherapy
Inflammatory Response
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
T Cell Types and Functions
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Cytotoxic T Cells-mediated Immune Response
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
Inhibitors of Viral Protein Synthesis
The Tumor Microenvironment

