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Related Experiment Video

Updated: Apr 22, 2026

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

Published on: February 6, 2018

Tabhu: tools for antibody humanization.

Pier Paolo Olimpieri¹, Paolo Marcatili¹, Anna Tramontano²

¹Department of Physics, Sapienza University, Rome 00185, Italy, Center for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark, Anker Engelunds Vej 1, 2800 Lyngby, Denmark and Istituto Pasteur-Fondazione Cenci Bolognetti, Rome 00185, Italy.

Bioinformatics (Oxford, England)

|October 12, 2014

Summary

Antibody humanization is crucial for safe clinical use. The Tabhu web server streamlines this process by providing tools for template selection, grafting, and affinity restoration, simplifying experimental workflows.

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Last Updated: Apr 22, 2026

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Area of Science:

Biotechnology
Immunology
Bioinformatics

Background:

Xenogeneic antibodies, while effective, can cause dangerous immune responses in humans.
Antibody humanization aims to improve safety while retaining antigen-binding capabilities.
Current humanization methods involve complex grafting and back-mutation experiments.

Purpose of the Study:

To present Tools for Antibody Humanization (Tabhu), a web server designed to aid in antibody humanization.
To provide a comprehensive suite of tools for critical steps in the humanization process.
To simplify and accelerate the experimental workflow for antibody humanization.

Main Methods:

Development of a web server integrating multiple antibody humanization tools.
Inclusion of functionalities for human template selection and CDR grafting.
Implementation of methods for back-mutation evaluation, antibody modeling, and structural analysis.

Main Results:

Tabhu offers a streamlined approach to antibody humanization.
The server integrates essential tools for template selection, grafting, and affinity restoration.
It assists users through critical experimental steps, reducing complexity and time.

Conclusions:

Tabhu provides a valuable resource for researchers and clinicians involved in antibody humanization.
The web server facilitates the production of safer, effective therapeutic antibodies.
It simplifies the complex process of antibody humanization, improving efficiency and outcomes.