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Updated: Apr 22, 2026
Human Blastocyst Biopsy and Vitrification
Published on: July 26, 2019
Unfolded protein response prevents blastocyst formation during preimplantation embryo development in vitro
Murat Basar1, Idil Bozkurt2, Ozlem Guzeloglu-Kayisli3
1Department of Obstetrics and Gynecology, Ohio State University College of Medicine, Columbus, Ohio; Department of Histology and Embryology, Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey.
Objective:
To study the effect of increased endoplasmic reticulum (ER) stress as a major nongenomic mechanism for arrested blastocyst development.
Design:
Cell and animal study.
Setting:
The Ohio State University and Yale University.
Animal(S):
Mice.
Intervention(S):
Pregnant mare serum gonadotropin and hCG were administered IP; two cell embryos were collected 48 hours after hCG administration.
Main Outcome Measure(S):
Blastocyst development rate.
Result(S):
No morphological difference was detected in control versus tunicamycin- (TM) treated embryos until the blastocyst stage. On day 4 of embryonic development, TM treatment reduced blastocyst formation from 79% to 4% and induced nuclear fragmentation. TM treatment caused 2-fold and 2.6-fold increase in binding immunoglobulin protein and spliced-X-box binding protein 1 mRNA expression, respectively. By comparison, the tauroursodeoxycholic acid + TM combination reversed the effect of TM alone on blastocyst formation to near control levels.
Conclusion(S):
These results indicate that increased ER stress during in vitro embryo development triggers an unfolded protein response (UPR) that negatively affects blastocyst formation and suggests that activation of UPR signaling may account for low rates of blastocyst development.
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