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Molecular characterization of metallo β-lactamase producing multidrug resistant Pseudomonas aeruginosa from various

Kalaivani Ramakrishnan1, Saranathan Rajagopalan, Shashikala Nair

  • 1Department of Clinical Microbiology, Pondicherry Institute of Medical Sciences, Puducherry, India.

Indian Journal of Pathology & Microbiology
|October 14, 2014
PubMed
Abstract

Insights

Metallo beta-lactamases (MBLs) are a predominant resistance mechanism in multidrug-resistant Pseudomonas aeruginosa (MDRPA). The blaVIM-2 gene was detected in 13% of MBL-producing MDRPA isolates, highlighting the need for effective antibiotic policies.

Area of Science:

  • Clinical microbiology
  • Molecular biology
  • Infectious diseases

Background:

  • * Pseudomonas aeruginosa* is a significant opportunistic pathogen, frequently causing severe hospital-acquired infections.
  • * Multidrug-resistant *P. aeruginosa* (MDRPA) poses a major clinical challenge due to limited treatment options.
  • * MDRPA employs various resistance mechanisms, including Metallo β-lactamase (MBL) production, extended-spectrum β-lactamase (ESBL) production, and altered outer membrane permeability.

Purpose of the Study:

  • * To investigate the predominant resistance mechanisms in MDRPA isolates.
  • * To determine the prevalence of specific resistance genes, particularly those encoding MBLs.

Main Methods:

  • * Phenotypic and molecular methods were used to analyze MDRPA isolates collected over one year.
  • * Polymerase chain reaction (PCR) was employed to detect the presence of *bla*VIM-2, *bla*IMP-1, *bla*OXA-23, and *bla*NDM-1 genes.

Main Results:

  • * 84% (63 out of 75) of MDRPA isolates were identified as MBL producers.
  • * The *bla*VIM-2 gene was detected in 13% of the MBL-producing isolates.

Conclusions:

  • * MBL production is a prevalent resistance mechanism in *P. aeruginosa*, contributing to therapeutic limitations.
  • * Strict antibiotic stewardship and effective resistance screening are crucial for managing MDRPA infections.

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