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Synthesis of an Intein-mediated Artificial Protein Hydrogel
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Hydrolytically degradable thiol-ene hydrogels for protein release.

Matthew S Rehmann1, Andrew C Garibian1, April M Kloxin2

  • 1Department of Chemical & Biomolecular Engineering, University of Delaware, 150 Academy Street, Colburn Laboratory, Newark, Delaware, U.S.A. 19716.

Macromolecular Symposia
|October 14, 2014
PubMed
Summary

A novel degradable polyethylene glycol (PEG)-ester-thiol hydrogel was developed for controlled protein release. This UV-curable hydrogel shows rapid polymerization, hydrolytic degradation over 3 weeks, and sustained release of bovine serum albumin (BSA).

Keywords:
degradationdrug delivery systemshydrogelsphotopolymerizationthiol–ene

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Area of Science:

  • Biomaterials Science
  • Polymer Chemistry
  • Drug Delivery Systems

Background:

  • Hydrogels are widely used in biomedical applications due to their tunable properties.
  • Developing degradable hydrogels with controlled release kinetics is crucial for therapeutic applications.
  • Photoinitiated polymerization offers rapid gelation and spatial control.

Purpose of the Study:

  • To synthesize and characterize a novel degradable polyethylene glycol (PEG)-ester-thiol hydrogel.
  • To evaluate the hydrogel's potential for controlled protein release.
  • To demonstrate a proof-of-concept for photoinitiated, hydrolytically degradable hydrogels for drug delivery.

Main Methods:

  • Synthesis of PEG-diester-dinorbornene and PEG-triester-trithiol monomers.
  • Photoinitiated step-growth polymerization using UV irradiation.
  • Hydrolytic degradation studies in aqueous buffer.
  • Encapsulation and in vitro release studies using bovine serum albumin (BSA) as a model protein.

Main Results:

  • The hydrogel rapidly polymerized within seconds under UV irradiation.
  • The hydrogel exhibited hydrolytic degradation over approximately 3 weeks in aqueous buffer.
  • Efficient encapsulation and sustained release of BSA were achieved, with ~90% released within 48 hours.
  • The study demonstrated the feasibility of creating hydrolytically degradable PEG-ester-thiol hydrogels via photoinitiation.

Conclusions:

  • A new class of hydrolytically degradable, PEG-ester-thiol hydrogels was successfully developed.
  • These hydrogels are suitable for controlled protein release applications.
  • Degradation and release kinetics can be tuned by adjusting monomer properties, offering potential for future therapeutic development.