Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Adenovirus phagocytosis by neutrophils triggers a pro-inflammatory response.

PLoS pathogens·2026
Same author

CHIASMA: Advancing chemicals and materials safety and sustainability assessments through innovative integration of in vitro and in silico (new approach) methodologies.

Computational and structural biotechnology journal·2025
Same author

Safe and Sustainable by Design MOF Beads for Selective Entrapment and Recovery of Rare Earth Elements.

Environmental science & technology·2025
Same author

CompSafeNano project: NanoInformatics approaches for safe-by-design nanomaterials.

Computational and structural biotechnology journal·2025
Same author

Assessing solvated electron uptake in low-temperature plasma-exposed solutions as a pathway to quantifying plasma electrons.

The Journal of chemical physics·2024
Same author

Quantifying hydroxyl radicals generated by a low-temperature plasma using coumarin: methodology and precautions.

Physical chemistry chemical physics : PCCP·2024

Related Experiment Video

Updated: Apr 22, 2026

Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials
07:54

Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials

Published on: October 27, 2020

4.0K

Could nanoparticle corona characterization help for biological consequence prediction?

Emilie Brun1, Cécile Sicard-Roselli1

  • 1Laboratoire de Chimie Physique, CNRS UMR8000, Université Paris-Sud, 91405 Orsay, Cedex France.

Cancer Nanotechnology
|October 14, 2014
PubMed
Summary

Nanoparticles interacting with biological fluids form a biomolecular corona, altering their properties and cellular interactions. Understanding this protein layer is crucial for predicting nanoparticle behavior in biological systems.

Keywords:
Cellular interactionCulture mediumGold nanoparticlesHydrodynamic sizeNanoparticle toxicityNanoparticle uptakeProtein coronaProtein identification

More Related Videos

Standardized SDS-PAGE Workflow for Personalized Protein Corona Profiling in Early Cancer Detection
10:02

Standardized SDS-PAGE Workflow for Personalized Protein Corona Profiling in Early Cancer Detection

Published on: December 19, 2025

799
Preparation of Nanoparticles for ToF-SIMS and XPS Analysis
06:24

Preparation of Nanoparticles for ToF-SIMS and XPS Analysis

Published on: September 13, 2020

9.7K

Related Experiment Videos

Last Updated: Apr 22, 2026

Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials
07:54

Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials

Published on: October 27, 2020

4.0K
Standardized SDS-PAGE Workflow for Personalized Protein Corona Profiling in Early Cancer Detection
10:02

Standardized SDS-PAGE Workflow for Personalized Protein Corona Profiling in Early Cancer Detection

Published on: December 19, 2025

799
Preparation of Nanoparticles for ToF-SIMS and XPS Analysis
06:24

Preparation of Nanoparticles for ToF-SIMS and XPS Analysis

Published on: September 13, 2020

9.7K

Area of Science:

  • Nanotechnology
  • Biomaterials Science
  • Cellular Biology

Background:

  • Nanoparticles in biological media acquire a biomolecular corona, primarily proteins.
  • This corona masks the original nanoparticle surface, influencing biological interactions.
  • Gold nanoparticles are a key focus for understanding corona formation and effects.

Purpose of the Study:

  • To review the formation and composition of the biomolecular corona on nanoparticles.
  • To analyze the impact of the corona on nanoparticle fate, including cellular uptake, toxicity, biodistribution, and targeting.
  • To emphasize the need for standardized experimental methods in nanoparticle research.

Main Methods:

  • Review of existing literature on nanoparticle-biomolecule interactions.
  • Analysis of factors influencing protein adsorption onto nanoparticle surfaces.
  • Synthesis of findings on the biological consequences of the biomolecular corona.

Main Results:

  • The biomolecular corona significantly alters nanoparticle size and protein composition.
  • Parameters influencing corona quantity and protein identity were identified.
  • The corona impacts cellular uptake, toxicity, biodistribution, and targeting efficacy.

Conclusions:

  • The biomolecular corona is a critical factor in nanoparticle biological behavior.
  • Standardized experimental reporting is essential for reliable data.
  • Incorporating corona effects into theoretical models is recommended for accurate nanomaterial representation.