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Maize U2 snRNAs: gene sequence and expression.

J W Brown1, R Waugh

  • 1Department of Biological Sciences, University of Dundee, UK.

Nucleic Acids Research
|November 25, 1989
PubMed
Summary
This summary is machine-generated.

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Plant splicing differences may stem from U-type small nuclear ribonucleoprotein particle (UsnRNPs) complexity. Maize U2snRNA genes show conserved secondary structures despite sequence variations, suggesting splicing differences aren't solely due to sequence changes.

Area of Science:

  • Molecular Biology
  • Plant Science
  • Genetics

Background:

  • Plant splicing mechanisms, particularly involving U-type small nuclear ribonucleoprotein particles (UsnRNPs), are complex and vary between plant groups.
  • Differences in splicing exist between monocotyledonous and dicotyledonous plants, suggesting underlying molecular distinctions.

Purpose of the Study:

  • To investigate the U2snRNA multigene family in maize (monocotyledonous) and compare it with dicotyledonous plants like Arabidopsis.
  • To explore potential sequence and structural variations in U2snRNAs that might explain differential splicing in plants.

Main Methods:

  • RNA blot analysis
  • RNase protection assays
  • Comparative sequence and secondary structure analysis of U2snRNA genes.

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Main Results:

  • The maize U2snRNA multigene family comprises 25-40 genes producing U2snRNAs with size and sequence variations.
  • Maize U2-27 snRNA shares identical sequences with Arabidopsis U2snRNA in the first 77 nucleotides.
  • Despite lower homology in the remaining sequence, the secondary structure of maize U2snRNA is conserved compared to Arabidopsis.

Conclusions:

  • Sequence differences in the potentially interacting regions of monocot and dicot U2snRNAs do not fully explain the observed splicing differences.
  • Conserved secondary structure of U2snRNA suggests that splicing variations may be influenced by other factors beyond simple sequence homology in the branch point interaction region.