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Updated: Apr 22, 2026

Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue

Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue

Published on: September 2, 2013

Proteomic developments in the analysis of formalin-fixed tissue.

Ove J R Gustafsson¹, Georgia Arentz¹, Peter Hoffmann¹

¹Adelaide Proteomics Centre, School of Molecular and Biomedical Science, The University of Adelaide, Adelaide, Australia 5005.

Biochimica Et Biophysica Acta

|October 16, 2014

Summary

Formalin-fixed tissue archives are crucial for retrospective cancer proteomic studies. This review details methods for analyzing proteins in these valuable formalin-fixed samples.

Keywords:

Cancer FFPE Formalin Proteomics Tissue Tumour

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Expanding the Comprehension of the Tumor Microenvironment using Mass Spectrometry Imaging of Formalin-Fixed and Paraffin-Embedded Tissue Samples

Expanding the Comprehension of the Tumor Microenvironment using Mass Spectrometry Imaging of Formalin-Fixed and Paraffin-Embedded Tissue Samples

Published on: June 29, 2022

Area of Science:

Biochemistry
Molecular Biology
Oncology

Background:

Retrospective proteomic studies require large patient tissue cohorts.
Accessing native tissue archives is challenging.
Formalin-fixed tissue archives offer a valuable resource for research.

Purpose of the Study:

To review current knowledge on formalin-fixed tissue for proteomic analysis.
To highlight methods applicable to archived tumor tissue.
To support molecular mechanism elucidation in cancer.

Main Methods:

Review of formalin fixation mechanisms.
Description of protein extraction techniques.
Overview of proteomics methods including top-down, bottom-up, quantitative, phospho-, glycoproteomics, and imaging mass spectrometry.

Main Results:

Formalin fixation preserves tissue but alters proteins.
Various proteomic techniques can be applied to formalin-fixed tissues.
Archived formalin-fixed tumor tissue is suitable for detailed proteomic investigation.

Conclusions:

Formalin-fixed tissue archives are a vital resource for retrospective cancer research.
Advances in proteomics enable comprehensive analysis of these samples.
This review provides a guide to proteomic methodologies for archived tissues.