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Related Concept Videos

MicroRNAs01:22

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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RNA Interference01:23

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
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Related Experiment Video

Updated: Mar 1, 2026

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery
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Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

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PLGA microspheres encapsulating siRNA.

Giuseppe De Rosa1, Giuseppina Salzano

  • 1Department of Pharmacy, Università degli Studi di Napoli Federico II, Via Domenico Montesano 49, 80131, Naples, Italy, gderosa@unina.it.

Methods in Molecular Biology (Clifton, N.J.)
|October 17, 2014
PubMed
Summary

Poly(lactide-co-glycolide) (PLGA) microspheres offer a promising solution for delivering small interfering RNA (siRNA) therapeutics. This method enhances siRNA stability and enables controlled, localized release, overcoming key delivery challenges for gene silencing applications.

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Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • RNA Therapeutics

Background:

  • Small interfering RNA (siRNA) therapeutics face challenges including short half-life, poor cellular uptake, and non-specific distribution.
  • Poly(lactide-co-glycolide) (PLGA) is a biodegradable and biocompatible polymer approved for parenteral administration, making it a suitable candidate for drug delivery systems.

Purpose of the Study:

  • To describe a protocol for preparing PLGA microspheres encapsulating siRNA.
  • To optimize the protocol for high siRNA encapsulation efficiency and sustained in vitro release.
  • To demonstrate the potential of PLGA microspheres for delivering various nucleic acid-based therapeutics.

Main Methods:

  • Double emulsion/solvent evaporation technique used for PLGA microsphere preparation.
  • Encapsulation of anti-TNFα siRNA within PLGA microspheres.
  • In vitro characterization of siRNA release kinetics.

Main Results:

  • Achieved high encapsulation efficiency for siRNA within PLGA microspheres.
  • Demonstrated sustained in vitro release of siRNA from the microspheres.
  • Established a reproducible method for creating siRNA-loaded PLGA microspheres.

Conclusions:

  • PLGA microspheres provide a protective and controlled delivery system for siRNA therapeutics.
  • The described protocol is adaptable for encapsulating various RNA and DNA-based oligonucleotides.
  • This approach addresses critical biopharmaceutical drawbacks of siRNA, advancing gene silencing therapies.