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Multiplex Detection and Quantification of miRNAs in Drug Delivery Systems Using a Signal-Off Electrochemical Platform
Wanda Cimmino1, Alessia Angelillo1, Panagiota M Kalligosfyri1
1Department of Pharmacy, University of Naples Federico II, Naples 80131, Italy.
Analytical Chemistry
|April 27, 2026
Summary
This study introduces a novel electrochemical biosensor for quantifying multiple microRNAs (miRNAs) within lipid nanoparticles (LNPs). This rapid, low-cost method enhances quality control for LNP-based therapeutics.
Area of Science:
- Analytical Chemistry
- Biotechnology
- Materials Science
Background:
- Lipid nanoparticles (LNPs) are crucial for delivering therapeutic microRNAs (miRNAs), but their quality control is challenging.
- Simultaneous quantification of multiple miRNAs in LNP formulations is a significant analytical hurdle.
Purpose of the Study:
- To develop a dual-working electrochemical biosensor for multiplexed, selective, and amplification-free quantification of miRNAs within LNPs.
- To address the analytical challenges in pharmaceutical quality control of miRNA-LNP formulations.
Main Methods:
- Fabrication of a biosensor on a dual-working screen-printed graphite electrode with gold electrodeposition and MB-labeled ss-DNA probes.
- Surfactant-mediated LNP lysis followed by hybridization-based signal-off detection using square wave voltammetry (SWV).
- Quantification of miR-4676 and miR-6503 in LNP formulations.
Main Results:
- Achieved limits of detection of 0.96 nM for miR-4676 and 0.98 nM for miR-6503.
- Demonstrated high selectivity and quantification accuracies of 88% (1:1 ratio) and 84% (2:1 ratio) in real formulations.
- Successfully enabled individual miRNA quantification in complex LNP mixtures.
Conclusions:
- The developed electrochemical platform offers a rapid, low-cost, and portable solution for multiplexed miRNA quantification in LNPs.
- This biosensor provides a viable tool for pharmaceutical quality control of advanced miRNA-based drug delivery systems.
