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RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

RNA Splicing

RNA Splicing

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Alternative RNA Splicing

Alternative RNA Splicing

Pre-mRNA Processing: RNA Splicing

Pre-mRNA Processing: RNA Splicing

Exon Recombination

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon...

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Related Experiment Video

Updated: Apr 21, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

TRAP150 activates splicing in composite terminal exons.

Kuo-Ming Lee¹, Woan-Yuh Tarn²

¹Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan.

Nucleic Acids Research

|October 19, 2014

Summary

This summary is machine-generated.

The spliceosomal factor TRAP150 regulates premature cleavage and polyadenylation (PCPA). Overexpressing TRAP150 activates cryptic splice sites, potentially increasing RNA transcript diversity during compromised gene expression.

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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Area of Science:

Molecular Biology
RNA Splicing
Gene Regulation

Background:

TRAP150 is a spliceosomal factor crucial for pre-mRNA splicing.
TRAP150 enhances splicing efficiency upon overexpression.
TRAP150 interacts with CPSF and RNA polymerase II, and associates with U1 snRNP.

Purpose of the Study:

To investigate the role of TRAP150 in premature cleavage and polyadenylation (PCPA).
To determine if TRAP150 acts as a cofactor in PCPA regulation.
To elucidate the mechanism by which TRAP150 influences splicing and PCPA.

Main Methods:

Co-immunoprecipitation to study protein interactions.
Cellular fractionation to assess co-localization.
TRAP150 depletion and overexpression studies.
Analysis of cryptic splice site activation and PCPA transcript formation.

Main Results:

TRAP150 overexpression induced the activation of cryptic 3' splice sites, leading to spliced PCPA transcripts.
TRAP150-activated splicing occurred in composite but not authentic terminal exons.
This activity was enhanced by U1 snRNP debilitation or interference with transcription elongation/termination.

Conclusions:

TRAP150 plays a regulatory role in PCPA, distinct from its general splicing functions.
TRAP150 can promote the generation of alternative RNA transcripts under specific cellular conditions.
TRAP150 may contribute to RNA transcript diversity during compromised gene expression.