[Cyclic changes of S100A10 expression in human endometrium].

Area of Science:

• Reproductive Biology
• Molecular Endocrinology
• Cell Biology

Background:

• S100A10 is a calcium-binding protein implicated in various cellular functions.
• Understanding its role in the endometrium is crucial for reproductive health.

Purpose of the Study:

• To investigate the dynamic changes in S100A10 gene and protein expression throughout the human menstrual cycle.
• To correlate S100A10 expression levels with specific phases of the endometrial cycle.

Main Methods:

• Real-time PCR was used to quantify S100A10 mRNA levels.
• Immunohistochemical staining and Western blot analysis assessed S100A10 protein localization and expression.
• Human endometrial samples were collected across different phases of the menstrual cycle.

Main Results:

• S100A10 protein is localized in the cytoplasm and cytomembrane of endometrial epithelial cells.
• Continuous expression of S100A10 was observed from the proliferative to the secretory phase.
• Significantly higher S100A10 protein and mRNA levels were detected in the secretory phase compared to the proliferative phase, with a peak in the mid-secretory phase.

Conclusions:

• Endometrial S100A10 expression exhibits significant cyclic variation, increasing during the secretory phase.
• Elevated S100A10 levels during the mid-secretory phase may play a role in preparing the endometrium for embryo implantation.
• S100A10 may contribute to enhanced endometrial receptivity, potentially by modulating uterine epithelial cell function.