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Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells
Published on: May 1, 2020
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Neuronal BC RNAs cooperate with eIF4B to mediate activity-dependent translational control
Taesun Eom1, Ilham A Muslimov1, Panayiotis Tsokas2
1Department of Physiology and Pharmacology, Department of Anesthesiology, and Department of Neurology, The Robert F. Furchgott Center for Neural and Behavioral Science, SUNY Downstate Medical Center, Brooklyn, NY 11203.
The Journal of Cell Biology
|October 22, 2014
Summary
Regulatory brain cytoplasmic RNAs and eukaryotic initiation factor 4B control neuronal translation. This mechanism, responsive to neuronal activity, impacts long-term changes in nerve cells.
Area of Science:
- Neuroscience
- Molecular Biology
- Cell Biology
Background:
- Translational regulation is crucial for activity-dependent neuronal function.
- Mechanisms of stimulus-modulated translational control in neurons are not fully understood.
Purpose of the Study:
- To elucidate a novel mechanism of translational control in neurons.
- To investigate the role of regulatory brain cytoplasmic (BC) RNAs and eukaryotic initiation factor 4B (eIF4B) in neuronal translation.
Main Methods:
- Investigated the interaction between BC RNAs and eIF4B in response to neuronal stimulation.
- Examined the phosphorylation status of eIF4B and its effect on translation initiation.
- Focused on the translation of neuronal protein kinase Mζ (PKMζ) mRNA.
Main Results:
- Neuronal stimulation leads to rapid dephosphorylation of eIF4B at serine 406 via protein phosphatase 2A.
- Dephosphorylated eIF4B exhibits reduced binding to BC RNA translational repressors.
- This facilitates eIF4B binding to the 40S ribosomal subunit, promoting translation initiation.
Conclusions:
- BC RNA-mediated translational control, regulated by eIF4B phosphorylation, links neuronal activity to protein synthesis.
- This provides a molecular basis for activity-dependent synaptic plasticity and long-term changes in neurons.
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