Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Deconvolution

Deconvolution

Deconvolution, also known as inverse filtering, is the process of extracting the impulse response from known input and output signals. This technique is vital in scenarios where the system's characteristics are unknown, and they must be inferred from the observable signals.
Deconvolution involves several mathematical techniques to derive the impulse response. One common approach is polynomial division. In this method, the input and output sequences are treated as coefficients of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Comparative study of triple intravenous chemotherapy versus dual chemotherapy combined with hepatic arterial infusion in patients with liver metastases from colorectal cancer.

Frontiers in oncology·2026

Same author

Fulminant extraneural metastases in MYC-amplified Group 3 medulloblastoma: a pediatric case report.

Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery·2026

Same author

Easily-wavelength-locked high-efficiency coupler-free external cavity spectral beam combining for diode lasers.

Optics express·2026

Same author

Hepatic SNHG9 links gut microbiota to liver protection in drug-induced liver injury.

Nature communications·2026

Same author

A Multifunctional Core-Shell Nanoemulsion-Mediated Disruption of Asphaltene Aggregates for Unconventional Reservoir Oil Recovery Enhancement.

Molecules (Basel, Switzerland)·2026

Same author

ZFP560 facilitates KAP1-dependent chromatin repression to regulate exit from totipotency.

Journal of genetics and genomics = Yi chuan xue bao·2026

Search research articles

Related Experiment Video

Updated: Apr 21, 2026

High-plex Imaging using Spectral Confocal Microscopy to Minimize Non-specific Tissue Fluorescence

High-plex Imaging using Spectral Confocal Microscopy to Minimize Non-specific Tissue Fluorescence

Published on: October 28, 2025

Shading correction for whole slide image using low rank and sparse decomposition.

Tingying Peng, Lichao Wang, Christine Bayer

Medical Image Computing and Computer-Assisted Intervention : MICCAI ... International Conference on Medical Image Computing and Computer-Assisted Intervention

|October 22, 2014

Summary

This study introduces novel algorithms to correct shading artifacts in whole slide images (WSI). These methods effectively remove illumination variations, improving image quality for digital pathology and microscopy analysis.

More Related Videos

Detection of Architectural Distortion in Prior Mammograms via Analysis of Oriented Patterns

Detection of Architectural Distortion in Prior Mammograms via Analysis of Oriented Patterns

Published on: August 30, 2013

Transient Optical Clearing Using Absorbing Molecules for Ex Vivo and In Vivo Imaging

Transient Optical Clearing Using Absorbing Molecules for Ex Vivo and In Vivo Imaging

Published on: July 11, 2025

Related Experiment Videos

Last Updated: Apr 21, 2026

High-plex Imaging using Spectral Confocal Microscopy to Minimize Non-specific Tissue Fluorescence

High-plex Imaging using Spectral Confocal Microscopy to Minimize Non-specific Tissue Fluorescence

Published on: October 28, 2025

Detection of Architectural Distortion in Prior Mammograms via Analysis of Oriented Patterns

Detection of Architectural Distortion in Prior Mammograms via Analysis of Oriented Patterns

Published on: August 30, 2013

Transient Optical Clearing Using Absorbing Molecules for Ex Vivo and In Vivo Imaging

Transient Optical Clearing Using Absorbing Molecules for Ex Vivo and In Vivo Imaging

Published on: July 11, 2025

Area of Science:

Microscopy and Digital Pathology
Image Processing and Computer Vision

Background:

Microscopic imaging often faces intensity inhomogeneity, known as shading artifacts, caused by uneven illumination or camera issues.
These artifacts, particularly visible as seams in whole slide images (WSI), hinder subsequent analyses like segmentation and registration.

Purpose of the Study:

To develop and validate two new retrospective shading correction algorithms for whole slide images (WSI).
To address common WSI formats: pre-mosaicked image tiles and already-stitched images.

Main Methods:

The proposed methods utilize matrix rank minimization and sparse signal recovery techniques.
Shading correction is reformulated as a low-rank and sparse component decomposition problem.
A sparse constraint in the Fourier domain ensures background illumination smoothness.

Main Results:

Extensive validation on synthetic and real microscopy images demonstrates superior shading removal performance.
The algorithms effectively separate foreground objects from background illumination fields.
Comparison with a well-established ImageJ method shows improved results.

Conclusions:

The developed algorithms offer effective retrospective shading correction for WSI.
These methods enhance image quality for critical downstream applications in digital pathology and microscopy.
The approach provides a robust solution for a common challenge in microscopic imaging.