Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Chromatin Modification in iPS Cells

Chromatin Modification in iPS Cells

Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...

Inheritance of Chromatin Structures

Inheritance of Chromatin Structures

Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying DNA...

Somatic to iPS Cell Reprogramming

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012 for this...

RNA Polymerase II Accessory Proteins

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...

Combinatorial Gene Control

Combinatorial Gene Control

Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...

Zygotic Development And Stem Cell Formation

Zygotic Development And Stem Cell Formation

The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

[Arthroscopic reconstruction of anterior cruciate ligament with preservation of the remnant bundle].

Zhongguo gu shang = China journal of orthopaedics and traumatology·2013

Same author

[Anterior cruciate ligament reconstruction with tendon graft enveloped by preserved remnants].

Zhongguo gu shang = China journal of orthopaedics and traumatology·2013

Same author

Genetic and molecular biological characterization of two homologous cheR genes from Leptospira interrogans.

Acta biochimica et biophysica Sinica·2013

Same author

Upregulation of glycoprotein nonmetastatic B by colony-stimulating factor-1 and epithelial cell adhesion molecule in hepatocellular carcinoma cells.

Oncology research·2013

Same author

Effect of implantation of biodegradable magnesium alloy on BMP-2 expression in bone of ovariectomized osteoporosis rats.

Materials science & engineering. C, Materials for biological applications·2013

Same author

[Texture variation of CC 5052 aluminum alloy slab from surface to center layer by XRD].

Guang pu xue yu guang pu fen xi = Guang pu·2013

Search research articles

Related Experiment Video

Updated: May 10, 2026

Zygotic Fluorescence Recovery After Photo-bleaching Analysis for Chromatin Looseness That Allows Full-term Development

Zygotic Fluorescence Recovery After Photo-bleaching Analysis for Chromatin Looseness That Allows Full-term Development

Published on: June 12, 2018

ZFP560 facilitates KAP1-dependent chromatin repression to regulate exit from totipotency.

Qingsheng Han¹, Lichao Wang¹, Chang Liu¹

¹School of Medicine, Nankai University, Tianjin 300071, China.

Journal of Genetics and Genomics = Yi Chuan Xue Bao

|May 8, 2026

Summary

Zinc finger protein 560 (ZFP560) regulates early embryonic development by maintaining heterochromatin structure. This KRAB-ZFP transcription factor is crucial for the exit from totipotency in mice.

Keywords:

2-Cell-like cells Embryonic stem cell KAP1 KRAB-ZFPs Pluripotency Totipotency

More Related Videos

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

Genome-wide Snapshot of Chromatin Regulators and States in Xenopus Embryos by ChIP-Seq

Genome-wide Snapshot of Chromatin Regulators and States in Xenopus Embryos by ChIP-Seq

Published on: February 26, 2015

Related Experiment Videos

Last Updated: May 10, 2026

Zygotic Fluorescence Recovery After Photo-bleaching Analysis for Chromatin Looseness That Allows Full-term Development

Zygotic Fluorescence Recovery After Photo-bleaching Analysis for Chromatin Looseness That Allows Full-term Development

Published on: June 12, 2018

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

Genome-wide Snapshot of Chromatin Regulators and States in Xenopus Embryos by ChIP-Seq

Genome-wide Snapshot of Chromatin Regulators and States in Xenopus Embryos by ChIP-Seq

Published on: February 26, 2015

Area of Science:

Epigenetics
Developmental Biology
Mammalian Embryogenesis

Background:

KRAB zinc finger proteins (KRAB-ZFPs) are key regulators of gene expression and epigenetic silencing in mammals.
They play a critical role in silencing transposable elements (TEs) during embryonic development.
The specific roles of early KRAB-ZFP effectors in embryonic development and cell state transitions are not well understood.

Purpose of the Study:

To investigate the role of zinc finger protein 560 (ZFP560) in early embryonic development and cell state transitions.
To elucidate the mechanism by which ZFP560 regulates the exit from totipotency.

Main Methods:

Identification of ZFP560 as a regulator of early embryonic development in mice.
Analysis of ZFP560's role in heterochromatin formation and KAP1 recruitment.
Assessment of ZFP560's impact on totipotency exit, MERVL expression, and 2-cell (2C) transcription factor activity.

Main Results:

ZFP560 is essential for safeguarding heterochromatin structure by recruiting KAP1, facilitating the exit from totipotency.
ZFP560 deficiency disrupts heterochromatin formation, halting the transition from totipotency to pluripotency.
ZFP560 overexpression promotes exit from the MERVL-positive state and suppresses 2C transcription factors by recruiting KAP1, reducing chromatin accessibility.

Conclusions:

ZFP560 is a highly expressed, 2C-specific KRAB-ZFP transcription factor.
ZFP560 acts as a mediator facilitating the exit from totipotency during early embryonic development.
These findings offer insights into the epigenetic regulation governing early embryonic development and cell state transitions.