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Updated: Apr 21, 2026
Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
Published on: January 5, 2021
NMR analysis of budding yeast metabolomics: a rapid method for sample preparation
C Airoldi1, F Tripodi, C Guzzi
1Department of Biotechnology and Biosciences, University of Milano-Bicocca, Milan, Italy. cristina.airoldi@unimib.it paola.coccetti@unimib.it.
Abstract:
Here we propose the optimization of a rapid and reproducible protocol for intracellular metabolite extraction from yeast cells and their metabolic profiling by (1)H-NMR spectroscopy. The protocol reliability has been validated through comparison between the metabolome of cells in different phases of growth or with different genetic backgrounds.
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