Related Experiment Video
Updated: Apr 21, 2026

HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis
Published on: November 11, 2022
[Study on HPLC fingerprint of Hedyotis chrysotricha]
Objective:
To establish HPLC fingerprint of Hedyotis chrysotricha.
Methods:
Hypersil BDS C18 (250 mm x 4.6 mm, 5 microm) column was used with methanol and 0.1% acetic acid solution as mobile phase in gradient mode, the flow rate was 1.0 mL/ min. The detection wavelength was 345 nm and the temperature of the column was set at 30 degrees C. Using ursolic acid as the reference standard, the mutual mode of Hedyotis chrysotricha containing nine common peaks was set up. The similarity of Hedyotis chrysotricha from different habitats was analyzed.
Results:
The fingerprints of Hedyotis chrysotricha from different habitats were similar, but also had habitat characteristics.
Conclusion:
The HPLC fingerprint can be used for identification and quality control of Hedyotis chrysotricha.
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