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3'-Arylazido-beta-alanyl-2-azido ATP, a cross-linking photoaffinity label for F1ATPases
H J Schäfer1, G Rathgeber, K Dose
1Institut für Biochemie, Johannes Gutenberg-Universität, Mainz, Bundesrepublik Deutschland.
Zeitschrift Fur Naturforschung. C, Journal of Biosciences
|November 1, 1989
Abstract:
The synthesis of the 3'-arylazido-2-azido ATP derivative 3'-O-(3-[N-(4-azido-2-nitrophenyl)-amino]propionyl)2-azido-adenosine 5'-triphosphate (2,3'-DiN3ATP) is described. The bifunctional photoreactive ATP analog is characterized spectroscopically. Photoaffinity labeling of F1ATPase from Micrococcus luteus by this analog results in the inactivation of the enzyme and in the formation of higher molecular weight cross-links, composed of alpha- and beta-subunits.