Related Experiment Videos
Convenient uses of polymerase chain reaction in analyzing recombinant cDNA clones
B K Nishikawa1, D M Fowlkes, B K Kay
1Dept. of Biology, University of North Carolina, Chapel Hill 27599-3280.
Biotechniques
|July 1, 1989
Abstract:
We have used polymerase chain reaction to accelerate our analysis of recombinant lambda-cDNA clones. We have amplified the inserts of lambda gt10 or lambda gt11 recombinants starting with bacteriophage in cored plaques or isolated DNA. The amplifications made with simple or complex oligonucleotide primers have allowed convenient sizing, subcloning and translation of the phage inserts into protein.