Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Sanger Sequencing01:57

Sanger Sequencing

799.9K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
799.9K
Next-generation Sequencing03:00

Next-generation Sequencing

87.2K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
87.2K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

10.3K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
10.3K
RNA-seq03:21

RNA-seq

9.1K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.1K
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

828
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
828
Genomic DNA in Prokaryotes00:46

Genomic DNA in Prokaryotes

42.1K
The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
42.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The mitogenome portrait of Umbria in Central Italy as depicted by contemporary inhabitants and pre-Roman remains.

Scientific reports·2020
Same author

Exploring the under-investigated "microbial dark matter" of drinking water treatment plants.

Scientific reports·2017
Same author

Complete mitochondrial sequences from Mesolithic Sardinia.

Scientific reports·2017
Same author

Corrigendum: A Unique Tryptophan C-Prenyltransferase from the Kawaguchipeptin Biosynthetic Pathway.

Angewandte Chemie (International ed. in English)·2017
Same author

Transcriptional, epigenetic and retroviral signatures identify regulatory regions involved in hematopoietic lineage commitment.

Scientific reports·2016
Same author

ICESpy009, a Conjugative Genetic Element Carrying mef(E) in Streptococcus pyogenes.

Antimicrobial agents and chemotherapy·2016
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Apr 21, 2026

Pyrosequencing for Microbial Identification and Characterization
12:37

Pyrosequencing for Microbial Identification and Characterization

Published on: August 22, 2013

48.5K

The pyrosequencing protocol for bacterial genomes.

Ermanno Rizzi1

  • 1National Research Council (CNR), Institute for Biomedical Technologies (ITB), Via F.lli Cervi 93, 20090, Segrate, MI, Italy, ermanno.rizzi@itb.cnr.it.

Methods in Molecular Biology (Clifton, N.J.)
|October 26, 2014
PubMed
Summary
This summary is machine-generated.

Pyrosequencing, a next-generation sequencing (NGS) method, offers high throughput and long reads for bacterial genome sequencing. This technology enables rapid de novo assembly and resequencing, delivering high-quality bacterial genomes efficiently.

More Related Videos

Pyrosequencing: A Simple Method for Accurate Genotyping
13:06

Pyrosequencing: A Simple Method for Accurate Genotyping

Published on: January 8, 2008

28.6K
Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
12:08

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

7.3K

Related Experiment Videos

Last Updated: Apr 21, 2026

Pyrosequencing for Microbial Identification and Characterization
12:37

Pyrosequencing for Microbial Identification and Characterization

Published on: August 22, 2013

48.5K
Pyrosequencing: A Simple Method for Accurate Genotyping
13:06

Pyrosequencing: A Simple Method for Accurate Genotyping

Published on: January 8, 2008

28.6K
Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
12:08

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

7.3K

Area of Science:

  • Genomics
  • Molecular Biology
  • Biotechnology

Background:

  • Next-generation sequencing (NGS) has transformed DNA sequencing capabilities.
  • Pyrosequencing, introduced by 454 Lifesciences in 2005, is a key NGS technology.
  • Technological advancements have increased pyrosequencing's throughput and read length.

Purpose of the Study:

  • To highlight the utility of pyrosequencing for bacterial genome analysis.
  • To demonstrate the effectiveness of pyrosequencing in de novo assembly and resequencing.
  • To showcase the speed and quality of results achievable with pyrosequencing.

Main Methods:

  • Application of pyrosequencing methodology.
  • Utilizing shotgun and paired-end sequencing approaches.
  • Technological updates enhancing throughput and read length (800-1,000 bases).

Main Results:

  • Pyrosequencing achieves high throughput for DNA sequencing.
  • Read lengths up to 800-1,000 bases are attainable.
  • Bacterial genome sequencing and finishing are accomplished in days with high-quality data.

Conclusions:

  • Pyrosequencing is well-suited for bacterial genome sequencing.
  • The technology facilitates efficient de novo assemblies and resequencing.
  • High-quality bacterial genomes can be rapidly obtained using pyrosequencing.