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Mitosis in Drosophila development.

D M Glover1, L Alphey, J M Axton

  • 1Department of Biochemistry, Imperial College of Science Technology and Medicine, London, UK.

Journal of Cell Science. Supplement
|January 1, 1989
PubMed
Summary
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In Drosophila embryos, DNA replication can occur without cell division, leading to giant nuclei. Centrosome replication and other cell cycle events proceed independently, even when DNA synthesis is blocked.

Area of Science:

  • Developmental Biology
  • Cell Biology
  • Genetics

Background:

  • The syncytial nature of early Drosophila embryos allows for independent progression of cell cycle events.
  • Mutations like gnu disrupt normal nuclear division, leading to DNA synthesis without cell division.
  • External agents like aphidicolin can inhibit DNA synthesis, revealing the independence of other mitotic processes.

Purpose of the Study:

  • To investigate the independence of various mitotic cell cycle events in Drosophila embryos.
  • To explore the behavior of centrosomes and nuclear structures when DNA synthesis is inhibited.
  • To examine the role of specific genes, such as cyclin B, in germ-line development.

Main Methods:

  • Utilizing the gnu mutation in Drosophila to study DNA replication without nuclear division.

Related Experiment Videos

  • Injecting aphidicolin into wild-type Drosophila embryos to inhibit S phase and observe subsequent events.
  • Analyzing centrosome behavior, nuclear envelope dynamics, and cortical activity.
  • Investigating the localization and accumulation of cyclin B mRNA in pole cells.

    • Main Results:

    • The gnu mutation results in DNA synthesis without nuclear division, producing giant nuclei and continued centrosome replication.
    • Aphidicolin injection inhibits DNA synthesis but allows centrosome replication, chromosome condensation/decondensation, nuclear envelope cycling, and cortical budding.
    • Inhibition of DNA synthesis can cause centrosome dissociation and migration, leading to pole cell formation without nuclei.
    • Cyclin B mRNA accumulates in pole cells, suggesting specific mitotic requirements for germ-line development.

    Conclusions:

    • Mitotic cell cycle events in Drosophila embryos exhibit significant independence.
    • Centrosome replication and other processes can proceed without concurrent DNA synthesis or nuclear division.
    • Specific gene products, like cyclin B, are crucial for the development of germ-line cells (pole cells).