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Related Concept Videos

Proteomics01:33

Proteomics

7.4K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

6.2K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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Updated: Apr 21, 2026

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames
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Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames

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Targeted proteomics: a bridge between discovery and validation.

Robert Harlan1, Hui Zhang

  • 1Department of Pathology, Johns Hopkins University, Baltimore, MD 21231, USA.

Expert Review of Proteomics
|October 29, 2014
PubMed
Summary
This summary is machine-generated.

New mass spectrometry technologies aid protein identification and quantitation. Targeted proteomics is crucial for validating potential diagnostic biomarkers, bridging discovery and clinical application.

Keywords:
LC–MSMRMPRMSRMbiomarkerproteomicstargeted proteomics

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Area of Science:

  • Proteomics
  • Biomarker Discovery
  • Mass Spectrometry

Background:

  • Clinical proteomics aims to identify diagnostic biomarkers.
  • Advancements in mass spectrometry offer new protein analysis capabilities.
  • Growing lists of potential biomarkers present validation challenges.

Purpose of the Study:

  • To highlight the role of emerging mass spectrometry technologies.
  • To emphasize the importance of targeted proteomics for biomarker validation.
  • To connect biomarker discovery with clinical assay development.

Main Methods:

  • Leveraging advanced mass spectrometry for protein identification and quantitation.
  • Employing high-throughput quantitative assays for biomarker verification.
  • Utilizing targeted proteomics approaches.

Main Results:

  • Maturing mass spectrometry technologies provide unique advantages.
  • Targeted proteomics enables efficient candidate biomarker validation.
  • The gap between biomarker discovery and clinical assays is being bridged.

Conclusions:

  • Mass spectrometry advancements are key to clinical proteomics.
  • Targeted proteomics is essential for translating biomarker discoveries into clinical tools.
  • Efficient validation is critical for developing reliable diagnostic assays.