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Compartmentalization of bacteria in microcapsules
Judith van Wijk1, Tiaan Heunis, Elrika Harmzen
1Eindhoven University of Technology, Department of Chemical Engineering and Chemistry, P.O. Box 513, 5600 MB Eindhoven University of Technology, The Netherlands.
Chemical Communications (Cambridge, England)
|October 30, 2014
Summary
Researchers developed a new method to encapsulate Lactobacillus plantarum using hollow poly(organosiloxane) microcapsules. This technique offers a promising approach for protecting microbial cells in various applications.
Area of Science:
- Biotechnology
- Materials Science
- Microbiology
Background:
- Microbial encapsulation is crucial for preserving cell viability and function.
- Developing robust and biocompatible microcapsules remains a challenge.
- Lactobacillus plantarum is a probiotic bacterium with significant industrial and health applications.
Purpose of the Study:
- To develop a novel method for encapsulating Lactobacillus plantarum strain 423.
- To create hollow poly(organosiloxane) microcapsules using Pickering emulsion templating.
- To ensure the viability of encapsulated microbial cells during the encapsulation process.
Main Methods:
- Utilized water-in-oil Pickering emulsion droplets as templates.
- Employed interfacial reaction of alkylchlorosilanes for microcapsule formation.
- Suspended Lactobacillus plantarum in growth medium or buffer to mitigate pH stress.
Main Results:
- Successfully encapsulated Lactobacillus plantarum strain 423 in hollow poly(organosiloxane) microcapsules.
- Demonstrated the protective effect of growth medium or buffer against pH changes during interfacial reactions.
- Achieved microcapsule formation via templating emulsion droplets.
Conclusions:
- The developed method provides a novel route for encapsulating microbial cells.
- Poly(organosiloxane) microcapsules offer a protective environment for Lactobacillus plantarum.
- This technique has potential applications in food, pharmaceutical, and biotechnological industries.
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