Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

25.3K
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
25.3K
RNA-seq03:21

RNA-seq

10.7K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.7K
RNA Interference01:23

RNA Interference

26.8K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.8K
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

17.4K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
17.4K
RNA Splicing01:32

RNA Splicing

58.0K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
58.0K
RNA Structure01:23

RNA Structure

75.7K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
75.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An examination of mindfulness on Mu suppression and pain empathy and its relation to trait empathy.

Social neuroscience·2023
Same author

Harmonizing Definitions for Diagnostic Criteria and Prognostic Assessment of Transplantation-Associated Thrombotic Microangiopathy: A Report on Behalf of the European Society for Blood and Marrow Transplantation, American Society for Transplantation and Cellular Therapy, Asia-Pacific Blood and Marrow Transplantation Group, and Center for International Blood and Marrow Transplant Research.

Transplantation and cellular therapy·2022
Same author

In reply to Huo et al.: Treating small hepatocellular carcinoma: Stereotactic body radiation therapy versus radiofrequency ablation.

Journal of gastroenterology and hepatology·2020
Same author

Dosimetric analysis of stereotactic rotational versus static intensity-modulated radiation therapy for pancreatic cancer.

Cancer radiotherapie : journal de la Societe francaise de radiotherapie oncologique·2018
Same author

Abdominal adiposity intensifies the negative effects of ambient air pollution on lung function in Korean men.

International journal of obesity (2005)·2017
Same author

Generation of RUNX3 knockout pigs using CRISPR/Cas9-mediated gene targeting.

Reproduction in domestic animals = Zuchthygiene·2016
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Oct 26, 2025

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA
07:24

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

Published on: July 9, 2021

2.5K

RNA-RNA SELEX.

B Cho1

  • 1Department of Applied Chemistry, Cheongju University, 298 Daeseongro, Cheongju Chungbuk, 360-764, S. Korea, brcho@cju.ac.kr.

Methods in Molecular Biology (Clifton, N.J.)
|October 30, 2014
PubMed
Summary
This summary is machine-generated.

This study details a method using affinity chromatography and gel electrophoresis to isolate specific RNA aptamers that bind to RNA stem-loop structures. The technique successfully yields a single, predominant RNA aptamer family for further research.

More Related Videos

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
11:34

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

6.8K
Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
09:04

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

9.7K

Related Experiment Videos

Last Updated: Oct 26, 2025

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA
07:24

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

Published on: July 9, 2021

2.5K
Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
11:34

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

6.8K
Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
09:04

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

9.7K

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • Systematic evolution of ligands by exponential enrichment (SELEX) is crucial for identifying RNA aptamers that bind to specific RNA structural motifs.
  • RNA aptamer isolation commonly employs affinity column chromatography and electrophoretic mobility shift assay (EMSA).

Purpose of the Study:

  • To describe a refined method for separating RNA aptamers that specifically bind to an RNA stem-loop target.
  • To achieve the isolation of a single predominant RNA aptamer family.

Main Methods:

  • Utilized affinity chromatography with a column pre-attached to the target RNA.
  • Employed nondenaturing polyacrylamide gel electrophoresis for high-resolution separation.
  • Applied the Systematic evolution of ligands by exponential enrichment (SELEX) protocol.

Main Results:

  • Successfully separated RNA aptamers exhibiting specific binding to the RNA stem-loop target.
  • Obtained a single predominant RNA aptamer family, indicating high specificity and purity.
  • Demonstrated the efficacy of the combined affinity chromatography and gel electrophoresis technique.

Conclusions:

  • The described method provides an effective approach for isolating specific RNA aptamers against RNA structural motifs.
  • This technique enhances the purity and yield of desired RNA aptamers, facilitating further functional studies.
  • The optimized protocol advances aptamer selection and characterization for molecular and therapeutic applications.