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Updated: Apr 21, 2026

Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules
Published on: April 25, 2025
Unravelling the conformations of di-(perylene bisimide acrylate) by combining time-resolved fluorescence-anisotropy
F Spreitler1, M Sommer, M Hollfelder
1Experimental Physics IV and BIMF, University of Bayreuth, D-95440 Bayreuth, Germany. juergen.koehler@uni-bayreuth.de.
Abstract:
We compare the results from time-resolved fluorescence anisotropy experiments and molecular modelling on perylene bisimide acrylate dimers which allows us to connect the observed spectral signatures unambiguously with the non-stacked and two (parallel and anti-parallel) stacked conformations. For the parallel stacked conformation the experimental data can be reproduced quantitatively using a model that assumes structural relaxation in the electronically excited state of the stacked aggregate. For the non-stacked conformation we find quantitative agreement between experiment and modelling only if a fast hopping of the electronic excitation between the perylene bisimide subunits is taken into account.
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